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Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2008 Mar 18; Vol. 127 (3-4), pp. 227-36. Date of Electronic Publication: 2007 Aug 21. - Publication Year :
- 2008
-
Abstract
- A novel proximity ligation assay (PLA) using a pan-serotype reactive monoclonal antibody was developed and evaluated for the detection of foot-and-mouth disease virus (FMDV) in clinical samples collected from field cases of disease. The FMDV-specific PLA was found to be 100 times more sensitive for virus detection than the commonly used antigen capture-ELISA (AgELISA). As few as five TCID50 were detected in individual assays, which was comparable with the analytical sensitivity of real-time RT-PCR. Although this assay was capable of detecting diverse isolates from all seven FMDV serotypes, the diagnostic sensitivity of the PLA assay was lower than real-time RT-PCR mainly due to a failure to detect some SAT 1, SAT 2 and SAT 3 FMDV strains. In conclusion, this new PLA format has high analytical sensitivity for the detection of FMDV in clinical samples and may prove valuable as a rapid and simple tool for use in FMD diagnosis.
- Subjects :
- Animals
Antibody Specificity
Enzyme-Linked Immunosorbent Assay methods
Enzyme-Linked Immunosorbent Assay veterinary
Foot-and-Mouth Disease virology
Immunoassay methods
RNA, Viral analysis
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction methods
Reverse Transcriptase Polymerase Chain Reaction veterinary
Sensitivity and Specificity
Serotyping
Time Factors
Antibodies, Monoclonal
Foot-and-Mouth Disease diagnosis
Foot-and-Mouth Disease Virus isolation & purification
Immunoassay veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1135
- Volume :
- 127
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17897794
- Full Text :
- https://doi.org/10.1016/j.vetmic.2007.08.026