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Simultaneous determination of amiloride and hydrochlorothiazide in human plasma by liquid chromatography/tandem mass spectrometry with positive/negative ion-switching electrospray ionisation.
- Source :
-
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2007; Vol. 21 (21), pp. 3427-34. - Publication Year :
- 2007
-
Abstract
- A new method for simultaneous determination of amiloride and hydrochlorothiazide by liquid chromatography/electrospray tandem mass spectrometry (LC/MS/MS) operated in positive and negative ionization switching mode was developed and validated. Protein precipitation with acetonitrile was selected for sample preparation. The analytes were separated on a Phenomenex Curosil-PFP (250x4.6 mm, 5 microm) column by a gradient elution with a mobile phase consisting of 0.15% formic acid solution containing 0.23% ammonium acetate and methanol pumped at a flow rate of 1.0 mL.min(-1). Rizatriptan was used as the internal standard (IS) for quantification. The determination was carried out on a Waters Quattro-micro triple-quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) mode using the following transitions monitored simultaneously: positive m/z 230-->171 for amiloride, m/z 270-->158 for rizatriptan, and negative m/z 296-->205 for hydrochlorothiazide. The lower limits of quantification (LLOQs) were 0.1 and 1.0 ng.mL(-1) for amiloride and hydrochlorothiazide, respectively, which were lower than other published methods by using ultraviolet (UV), fluorimetric or mass spectrometric detection. The intra- and inter-day precision and accuracy were studied at three different concentration levels and were always better than 15% (n=5). This simple and robust LC/MS/MS method was successfully applied to the pharmacokinetic study of compound amiloride and hydrochlorothiazide tablets in healthy male Chinese volunteers.<br /> (Copyright (c) 2007 John Wiley & Sons, Ltd.)
- Subjects :
- Administration, Oral
Amiloride pharmacokinetics
Chromatography, High Pressure Liquid
Diuretics pharmacokinetics
Drug Therapy, Combination
Humans
Hydrochlorothiazide pharmacokinetics
Male
Reproducibility of Results
Serotonin Receptor Agonists blood
Triazoles blood
Tryptamines blood
Amiloride blood
Diuretics blood
Hydrochlorothiazide blood
Spectrometry, Mass, Electrospray Ionization methods
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 0951-4198
- Volume :
- 21
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Rapid communications in mass spectrometry : RCM
- Publication Type :
- Academic Journal
- Accession number :
- 17902196
- Full Text :
- https://doi.org/10.1002/rcm.3235