Nisin-controlled extracellular production of interleukin-2 in Lactococcus lactis strains, without the requirement for a signal peptide sequence.

Authors :: Fernández A
Rodríguez JM
Bongaerts RJ
Gasson MJ
Horn N
Source :: Applied and environmental microbiology [Appl Environ Microbiol] 2007 Dec; Vol. 73 (23), pp. 7781-4. Date of Electronic Publication: 2007 Sep 28.
Publication Year :: 2007
Abstract: Secretion of the cytokine interleukin-2 (IL-2) was investigated in Lactococcus lactis using the secretory machinery of the bacteriocin lactococcin A. Surprisingly, the lcnCD transport genes were not essential for mouse IL-2 secretion. Furthermore, expression of a mature mouse IL-2 gene resulted in interleukin secretion without the requirement for a leader sequence.

Subjects :: Animals
Bacteriocins genetics
Bacteriocins metabolism
Blotting, Western
L-Lactate Dehydrogenase genetics
L-Lactate Dehydrogenase metabolism
Lactococcus lactis genetics
Mice
Promoter Regions, Genetic genetics
Interleukin-2 metabolism
Lactococcus lactis metabolism
Nisin genetics
Protein Sorting Signals genetics

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