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Non-invasive in vivo imaging of calcium signaling in mice.
- Source :
-
PloS one [PLoS One] 2007 Oct 03; Vol. 2 (10), pp. e974. Date of Electronic Publication: 2007 Oct 03. - Publication Year :
- 2007
-
Abstract
- Rapid and transient elevations of Ca(2+) within cellular microdomains play a critical role in the regulation of many signal transduction pathways. Described here is a genetic approach for non-invasive detection of localized Ca(2+) concentration ([Ca(2+)]) rises in live animals using bioluminescence imaging (BLI). Transgenic mice conditionally expressing the Ca(2+)-sensitive bioluminescent reporter GFP-aequorin targeted to the mitochondrial matrix were studied in several experimental paradigms. Rapid [Ca(2+)] rises inside the mitochondrial matrix could be readily detected during single-twitch muscle contractions. Whole body patterns of [Ca(2+)] were monitored in freely moving mice and during epileptic seizures. Furthermore, variations in mitochondrial [Ca(2+)] correlated to behavioral components of the sleep/wake cycle were observed during prolonged whole body recordings of newborn mice. This non-invasive imaging technique opens new avenues for the analysis of Ca(2+) signaling whenever whole body information in freely moving animals is desired, in particular during behavioral and developmental studies.
- Subjects :
- Animals
Animals, Newborn
Cytosol metabolism
Diagnostic Imaging instrumentation
Genes, Reporter
Green Fluorescent Proteins metabolism
Mice
Mice, Transgenic
Models, Biological
Muscle Contraction
Neurons metabolism
Oscillometry
Signal Transduction
Calcium metabolism
Calcium Signaling
Diagnostic Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 2
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 17912353
- Full Text :
- https://doi.org/10.1371/journal.pone.0000974