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MicroRNA expression profiling of human breast cancer identifies new markers of tumor subtype.
- Source :
-
Genome biology [Genome Biol] 2007; Vol. 8 (10), pp. R214. - Publication Year :
- 2007
-
Abstract
- Background: MicroRNAs (miRNAs), a class of short non-coding RNAs found in many plants and animals, often act post-transcriptionally to inhibit gene expression.<br />Results: Here we report the analysis of miRNA expression in 93 primary human breast tumors, using a bead-based flow cytometric miRNA expression profiling method. Of 309 human miRNAs assayed, we identify 133 miRNAs expressed in human breast and breast tumors. We used mRNA expression profiling to classify the breast tumors as luminal A, luminal B, basal-like, HER2+ and normal-like. A number of miRNAs are differentially expressed between these molecular tumor subtypes and individual miRNAs are associated with clinicopathological factors. Furthermore, we find that miRNAs could classify basal versus luminal tumor subtypes in an independent data set. In some cases, changes in miRNA expression correlate with genomic loss or gain; in others, changes in miRNA expression are likely due to changes in primary transcription and or miRNA biogenesis. Finally, the expression of DICER1 and AGO2 is correlated with tumor subtype and may explain some of the changes in miRNA expression observed.<br />Conclusion: This study represents the first integrated analysis of miRNA expression, mRNA expression and genomic changes in human breast cancer and may serve as a basis for functional studies of the role of miRNAs in the etiology of breast cancer. Furthermore, we demonstrate that bead-based flow cytometric miRNA expression profiling might be a suitable platform to classify breast cancer into prognostic molecular subtypes.
- Subjects :
- Argonaute Proteins
Breast Neoplasms classification
DEAD-box RNA Helicases metabolism
DNA Primers
Endoribonucleases metabolism
Eukaryotic Initiation Factor-2 metabolism
Female
Flow Cytometry
Humans
MicroRNAs genetics
Ribonuclease III
Breast Neoplasms metabolism
Gene Expression Profiling methods
Gene Expression Regulation
Mammary Glands, Human metabolism
MicroRNAs metabolism
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1474-760X
- Volume :
- 8
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Genome biology
- Publication Type :
- Academic Journal
- Accession number :
- 17922911
- Full Text :
- https://doi.org/10.1186/gb-2007-8-10-r214