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Immunoadsorption and subsequent immunoglobulin substitution decreases myocardial gene expression of desmin in dilated cardiomyopathy.
- Source :
-
Journal of molecular medicine (Berlin, Germany) [J Mol Med (Berl)] 2007 Dec; Vol. 85 (12), pp. 1429-35. Date of Electronic Publication: 2007 Oct 09. - Publication Year :
- 2007
-
Abstract
- Cardiac autoantibodies play a pathogenic role in dilated cardiomyopathy (DCM). Removal of antibodies by immunoadsorption (IA) induces hemodynamic improvement in DCM patients. The present study investigated the effects of IA on myocardial gene expression of the intermediate cytoskeletal filament desmin, which is upregulated in heart failure. RNA was isolated from five explanted non-failing hearts and five explanted failing hearts of DCM patients, and myocardial gene expression of desmin was estimated by real-time polymerase chain reaction (PCR). In a case-control study in six DCM patients (LVEF < 40%, NYHA II-III), IA and subsequent IgG substitution were performed at monthly intervals until month 3. Endomyocardial biopsies (EMBs) were obtained before and after IA (after 3-6 months). From six DCM patients without IA therapy (controls), EMBs were also obtained over a comparable time interval. Expression of the desmin gene was analyzed in these EMBs by real-time PCR. In failing explanted hearts, expression of desmin was significantly increased (0.88 +/- 0.12 vs 0.45 +/- 0.15 in non-failing hearts, P < 0.05). After IA, myocardial gene expression of desmin was significantly decreased (from 0.26 +/- 0.05 [baseline] to 0.14 +/- 0.04 [P < 0.05] vs baseline and controls). Removal of antibodies by IA modulates myocardial gene expression of desmin in DCM patients.
- Subjects :
- Adult
Cardiomyopathy, Dilated genetics
Cardiomyopathy, Dilated immunology
Cardiomyopathy, Dilated metabolism
Case-Control Studies
Desmin genetics
Female
Humans
Male
Middle Aged
Pilot Projects
RNA, Messenger metabolism
Research Design
Time Factors
Tissue Culture Techniques
Autoantibodies blood
Cardiomyopathy, Dilated therapy
Desmin metabolism
Gene Expression Regulation
Immunoglobulins, Intravenous therapeutic use
Immunosorbent Techniques
Myocardium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0946-2716
- Volume :
- 85
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of molecular medicine (Berlin, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 17924085
- Full Text :
- https://doi.org/10.1007/s00109-007-0263-5