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Molecular characterization of a transient expression gene encoding for 1-aminocyclopropane-1-carboxylate synthase in cotton (Gossypium hirsutum L.).
- Source :
-
Journal of biochemistry and molecular biology [J Biochem Mol Biol] 2007 Sep 30; Vol. 40 (5), pp. 791-800. - Publication Year :
- 2007
-
Abstract
- Ethylene performs an important function in plant growth and development. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS), the key enzyme involved in ethylene biosynthesis, has been the focus of most ethylene studies. Here, a cotton ACS gene referred to as Gossypium hirsutum ACS1 (GhACS1), was isolated. The full-length cDNA of GhACS1 encodes for a 476-amino acid protein which harbors seven conserved regions, 11 invariant amino acid residues, and the PLP binding active site, all of which characterize ACC synthases. Alignment analysis showed that GhACS1 shared a high degree of identity with other known ACC synthases from different species. Two introns were detected in the genomic DNA sequence, and the results of Southern blot analysis suggested that there might be a multi-gene family encoding for ACC synthase in cotton. From the phylogenetic tree constructed with 24 different kinds of ACC synthases, we determined that GhACS1 falls into group II, and was closely associated with the wound-inducible ACS of citrus. The analysis of the 5' flanking region of GhACS1 revealed a group of putative cis-acting elements. The results of expression analysis showed that GhACS1 displayed its transient expression nature after wounding, abscisic acid (ABA), and CuCl(2) treatments. These results indicate that GhACS1, which was transiently expressed in response to certain stimuli, may be involved in the production of ethylene for the transmission of stress signals.
- Subjects :
- 5' Flanking Region genetics
Abscisic Acid pharmacology
Amino Acid Sequence
Base Sequence
Blotting, Southern
Cloning, Molecular
Copper pharmacology
DNA, Complementary chemistry
DNA, Complementary genetics
Gene Expression Regulation, Enzymologic drug effects
Gene Expression Regulation, Plant drug effects
Molecular Sequence Data
Phylogeny
Plant Growth Regulators pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Gene Expression Profiling
Gossypium genetics
Lyases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1225-8687
- Volume :
- 40
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 17927914
- Full Text :
- https://doi.org/10.5483/bmbrep.2007.40.5.791