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Influence of L-methioninase targeted to the urokinase receptor on the proliferation and motility of lung and prostate cancer cells.
- Source :
-
Anticancer research [Anticancer Res] 2007 Sep-Oct; Vol. 27 (5A), pp. 3435-9. - Publication Year :
- 2007
-
Abstract
- Background: Previously, we reported that a novel fusion protein consisting of an amino-terminal fragment of urokinase linked to the amino terminus of the enzyme L-methioninase inhibited MCF-7 breast cancer cells in vitro to a greater extent than treatment with L-methioninase.<br />Materials and Methods: The fusion protein, L-methioninase and a mutated fusion protein without L-methioninase activity were produced by recombinant methods. The effects of fusion protein, L-methioninase, and mutated fusion protein treatment on the proliferation and motility of SK-LU-i lung and PC-3 prostate and cancer cells were measured in vitro using a culture wounding assay.<br />Results: The fusion protein produced a dose-dependent inhibition of the proliferation and motility of both cancer cell lines. In addition, the fusion protein was found to be significantly more effective than L-methioninase alone or mutated fusion protein.<br />Conclusion: Our results suggest that this fusion protein has potential as a selective therapeutic agent for the treatment of various methionine-dependent cancers.
- Subjects :
- Carbon-Sulfur Lyases genetics
Cell Growth Processes drug effects
Cell Growth Processes physiology
Cell Line, Tumor
Cell Movement drug effects
Cell Movement physiology
Drug Delivery Systems
Humans
Lung Neoplasms metabolism
Male
Mutation
Peptide Fragments genetics
Peptide Fragments pharmacology
Prostatic Neoplasms metabolism
Receptors, Cell Surface genetics
Receptors, Urokinase Plasminogen Activator
Recombinant Fusion Proteins genetics
Carbon-Sulfur Lyases pharmacology
Lung Neoplasms drug therapy
Lung Neoplasms pathology
Prostatic Neoplasms drug therapy
Prostatic Neoplasms pathology
Receptors, Cell Surface metabolism
Recombinant Fusion Proteins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0250-7005
- Volume :
- 27
- Issue :
- 5A
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 17970092