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Production of monoclonal antibodies against Neisseria meningitidis using popliteal lymph nodes and in vivo/in vitro immunization: prevalence study of new monoclonal antibodies in greater São Paulo, Brazil.
- Source :
-
Hybridoma (2005) [Hybridoma (Larchmt)] 2007 Oct; Vol. 26 (5), pp. 302-10. - Publication Year :
- 2007
-
Abstract
- A rapid and efficient method for preparing monoclonal antibody (MAb) serotypes using Neisseria meningitidis outer membrane were used in BALB/c mouse footpads for the immunization. The popliteal lymph nodes were isolated 19 days later for MAb-producing hybridomas, from which the MAbs against the 37 kDa protein were screened. Variations in class 2/3 (PorB) proteins form the basis for meningococcal serotyping. This is the first report on the preparation of MAbs against N. meningitidis that is specific to PorB protein using popliteal lymph nodes. The new monoclonal antibodies were specific for PorB outer membrane protein FL24(PL)Br, a new serotype 24 class 3 antigens of non-typeable (NT:NST) serogroup B strain, and FL14(PL)Br specific for the serotype 14, and reacted with the S3446 reference strain analyzed. A total of 12% of the case isolates reacted with one or more of the monoclonal antibodies. The high-affinity MAbs produced by hybridoma methodology provide a basis for further research on the pathogenesis and early diagnosis of meningococcus.
- Subjects :
- Animals
Antibodies, Bacterial therapeutic use
Antibodies, Monoclonal therapeutic use
Bacteremia epidemiology
Bacteremia immunology
Bacteremia therapy
Brazil
Female
Humans
Lymph Nodes immunology
Meningococcal Infections epidemiology
Meningococcal Infections immunology
Meningococcal Infections therapy
Mice
Mice, Inbred BALB C
Prevalence
Antibodies, Bacterial biosynthesis
Antibodies, Monoclonal biosynthesis
Immunization
Lymph Nodes metabolism
Neisseria meningitidis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1554-0014
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Hybridoma (2005)
- Publication Type :
- Academic Journal
- Accession number :
- 17979546
- Full Text :
- https://doi.org/10.1089/hyb.2007.0508