[Clone of HPV58 E6 gene and analysis of HLA-DQB1 03 restricted T-cell epitopes].

Aim: To clone HPV58 E6 gene and analyze the HLA-DQB1 03-restricted T cell epitopes on E6 protein.
Methods: Total genome DNA was isolated from a cervical cancer sample. The HPV58 E6 gene was amplified by PCR and inserted into pGEM-T Easy vector. The recombinant plasmid was identified by restriction endonuclease analysis and sequencing. HLA-DQB1 03-restricted T cell epitopes on E6 protein were predicted and analyzed by the position-specific scoring matrix, support vector machine theory and prediction algorithm for proteasomal cleavages.
Results: A HPV58 E6 gene was successfully cloned and submitted to GenBank (EF060239). Epitope 47(FADLRIAYRDGNPFA) and Epitope 102(RCIICQRPLCPQEKK) were theoretic HLA-DQB1 03-restricted T cell epitopes on E6 protein.
Conclusion: These two epitopes could serve as candidates for screening and identification of the vaccine against HPV58 infection in the further study.