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The temporal sequence of gut peptide CNS interactions tracked in vivo by magnetic resonance imaging.

Authors :
Kuo YT
Parkinson JR
Chaudhri OB
Herlihy AH
So PW
Dhillo WS
Small CJ
Bloom SR
Bell JD
Source :
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2007 Nov 07; Vol. 27 (45), pp. 12341-8.
Publication Year :
2007

Abstract

Hormonal satiety signals secreted by the gut play a pivotal role in the physiological control of appetite. However, therapeutic exploitation of the gut-brain axis requires greater insight into the interaction of gut hormones with CNS circuits of appetite control. Using the manganese ion (Mn2+) as an activity-dependent magnetic resonance imaging (MRI) contrast agent, we showed an increase in signal intensity (SI) in key appetite-regulatory regions of the hypothalamus, including the arcuate, paraventricular, and ventromedial nuclei, after peripheral injection of the orexigenic peptide ghrelin. Conversely, administration of the anorexigenic hormone peptide YY(3-36) caused a reduction in SI. In both cases, the changes in SI recorded in the hypothalamic arcuate nucleus preceded the effect of these peptides on food intake. Intravenous Mn2+ itself did not significantly alter ghrelin-mediated expression of the immediate early gene product c-Fos, nor did it cause abnormalities of behavior or metabolic parameters. We conclude that manganese-enhanced MRI constitutes a powerful tool for the future investigation of the effects of drugs, hormones, and environmental influences on neuronal activity.

Details

Language :
English
ISSN :
1529-2401
Volume :
27
Issue :
45
Database :
MEDLINE
Journal :
The Journal of neuroscience : the official journal of the Society for Neuroscience
Publication Type :
Academic Journal
Accession number :
17989298
Full Text :
https://doi.org/10.1523/JNEUROSCI.2391-07.2007