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Rapid determination of hazardous compounds in food based on a competitive fluorescence microsphere immunoassay.
- Source :
-
Analytical biochemistry [Anal Biochem] 2008 Mar 15; Vol. 374 (2), pp. 318-24. Date of Electronic Publication: 2007 Oct 24. - Publication Year :
- 2008
-
Abstract
- Development of a microsphere-based competitive fluorescence immunoassay for the determination of hazardous low-molecular-weight compounds in food is described. In this method, antigens are covalently bound to carboxy-modified microspheres to compete monoclonal antibody with low-molecular-weight compounds in food samples; mouse IgG/fluorescein isothiocyanate conjugate is used as the fluorescent molecular probe. Thus, the hazardous low-molecular-weight compounds are quantified using a multiparameter flow cytometer. This method has been evaluated using clenbuterol as a model compound. It has a sensitivity of 0.01 ng/mL with dynamic range of 0.01-100 ng/mL, and the concentration of clenbuterol providing 50% inhibition (IC(50)) is 1.1 ng/mL. The main advantages of this method are its high efficiency, biocompatibility, and selectivity, as well as ultralow trace sample consumption and low cost.
Details
- Language :
- English
- ISSN :
- 1096-0309
- Volume :
- 374
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 18023398
- Full Text :
- https://doi.org/10.1016/j.ab.2007.10.024