Back to Search Start Over

Adeno-associated virus type 12 (AAV12): a novel AAV serotype with sialic acid- and heparan sulfate proteoglycan-independent transduction activity.

Authors :
Schmidt M
Voutetakis A
Afione S
Zheng C
Mandikian D
Chiorini JA
Source :
Journal of virology [J Virol] 2008 Feb; Vol. 82 (3), pp. 1399-406. Date of Electronic Publication: 2007 Nov 28.
Publication Year :
2008

Abstract

Recombinant adeno-associated virus (rAAV) is a promising vector for gene therapy. Recent isolations of novel AAV serotypes have led to significant advances by broadening the tropism and increasing the efficiency of gene transfer to the desired target cell. However, a major concern that remains is the strong preexisting immune responses to several vectors. In this paper, we describe the isolation and characterization of AAV12, an AAV serotype with unique biological and immunological properties. In contrast to those of all other reported AAVs, AAV12 cell attachment and transduction do not require cell surface sialic acids or heparan sulfate proteoglycans. Furthermore, rAAV12 is resistant to neutralization by circulating antibodies from human serum. The feasibility of rAAV12 as a vector was demonstrated in a mouse model in which muscle and salivary glands were transduced. These characteristics make rAAV12 an interesting candidate for gene transfer applications.

Details

Language :
English
ISSN :
1098-5514
Volume :
82
Issue :
3
Database :
MEDLINE
Journal :
Journal of virology
Publication Type :
Academic Journal
Accession number :
18045941
Full Text :
https://doi.org/10.1128/JVI.02012-07