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Adeno-associated virus type 12 (AAV12): a novel AAV serotype with sialic acid- and heparan sulfate proteoglycan-independent transduction activity.
- Source :
-
Journal of virology [J Virol] 2008 Feb; Vol. 82 (3), pp. 1399-406. Date of Electronic Publication: 2007 Nov 28. - Publication Year :
- 2008
-
Abstract
- Recombinant adeno-associated virus (rAAV) is a promising vector for gene therapy. Recent isolations of novel AAV serotypes have led to significant advances by broadening the tropism and increasing the efficiency of gene transfer to the desired target cell. However, a major concern that remains is the strong preexisting immune responses to several vectors. In this paper, we describe the isolation and characterization of AAV12, an AAV serotype with unique biological and immunological properties. In contrast to those of all other reported AAVs, AAV12 cell attachment and transduction do not require cell surface sialic acids or heparan sulfate proteoglycans. Furthermore, rAAV12 is resistant to neutralization by circulating antibodies from human serum. The feasibility of rAAV12 as a vector was demonstrated in a mouse model in which muscle and salivary glands were transduced. These characteristics make rAAV12 an interesting candidate for gene transfer applications.
- Subjects :
- Animals
Antibodies, Viral
Dependovirus immunology
Dependovirus isolation & purification
Genetic Vectors
Haplorhini
Heparitin Sulfate physiology
Humans
Male
Mice
Molecular Sequence Data
N-Acetylneuraminic Acid physiology
Neutralization Tests
Parvoviridae Infections virology
Sequence Analysis, DNA
Viral Proteins genetics
Dependovirus classification
Dependovirus physiology
Transduction, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 82
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 18045941
- Full Text :
- https://doi.org/10.1128/JVI.02012-07