Back to Search
Start Over
Mucosal priming with replicative Tiantan vaccinia and systemic boosting with DNA vaccine raised strong mucosal and systemic HIV-specific immune responses.
- Source :
-
Vaccine [Vaccine] 2007 Dec 17; Vol. 25 (52), pp. 8874-84. Date of Electronic Publication: 2007 Sep 24. - Publication Year :
- 2007
-
Abstract
- An effective vaccine strategy for HIV-1 will probably require the induction and maintenance of both humoral and cellular immunity at mucosal and systemic sites. We tested a new prime-boost approach of intranasal priming with 3 x 10(6) PFU of replicative recombinant Tiantan vaccinia virus (rTTV) and intramuscular boosting with 100 microg DNA plasmid expressing HIV-1 Gag in BALB/c mice along with other strategies. Our data demonstrated that intranasal priming with replicative recombinant Tiantan vaccinia and intramuscular boosting with DNA vaccine raised the highest vaginal IgA and systemic T-cell responses, and modest lung IgA and sera IgG responses among all vaccination regimens; each vaccination regimen generated its own imprint of the most preferential T-cell receptor usage of Vbeta. These results demonstrate that the combination of intranasal priming with replicative recombinant Tiantan vaccinia and intramuscular boosting with DNA vaccine is a preferable regimen for induction of both T-cell and humoral immune responses at mucosal and systemic sites.
- Subjects :
- AIDS Vaccines genetics
Administration, Intranasal
Animals
Female
HIV Antibodies analysis
HIV-1 genetics
Humans
Immunity, Mucosal
Immunization, Secondary
Immunoglobulin A analysis
Immunoglobulin G blood
Injections, Intramuscular
Lung immunology
Mice
T-Lymphocytes immunology
Vaccines, DNA administration & dosage
Vaccines, DNA genetics
Vaccinia virus genetics
Vagina immunology
Viral Vaccines administration & dosage
Viral Vaccines genetics
gag Gene Products, Human Immunodeficiency Virus genetics
AIDS Vaccines immunology
HIV-1 immunology
Vaccines, DNA immunology
Vaccinia virus immunology
Viral Vaccines immunology
gag Gene Products, Human Immunodeficiency Virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 25
- Issue :
- 52
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 18061316
- Full Text :
- https://doi.org/10.1016/j.vaccine.2007.08.066