Back to Search
Start Over
Microlipid-induced oxidative stress in human breastmilk: in vitro effects on intestinal epithelial cells.
- Source :
-
Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine [Breastfeed Med] 2007 Dec; Vol. 2 (4), pp. 209-18. - Publication Year :
- 2007
-
Abstract
- Objectives: To (1) determine whether medium chain fatty acids (Microlipid) added to human breastmilk generates reactive oxygen species (ROS), and (2) measure the physiological effect(s) of Microlipid) (ML)-supplemented human breastmilk in an enterocyte cell culture bioassay.<br />Methods: ML was added to milk according to manufacturer's recommendations and total hydroperoxides measured at intervals with the FOX 2 and TBARS assays. Physiological effects of supplementation were measured using a human enterocyte cell line (Caco-2BBE) and/or a primary human fetal intestinal cell culture (FHS-74 Int). Endpoints included: intracellular oxidative stress, transepithelial electrical resistance (TEER), apoptosis, and interleukin (IL)-6 production.<br />Results: Immediately postsupplementation, ML did not significantly increase ROS, as determined by both the FOX 2 and TBARS assays. Further, storage of milk + ML at 4 degrees C prevented significant increases in total hydroperoxides. However, by 4 hours postsupplementation at room temperature, both assays revealed significantly higher hydroperoxide and lipid peroxide levels. ML-supplemented milk stored at room temperature for 4 hours had the following effects in cell culture bioassays: elevated oxidative stress, increased rates of apoptosis, decreased transmembrane electrical resistance (TEER) values and, in both cell culture assays, significantly increased secretion of IL-6.<br />Conclusions: Based on our measurements of extracellular and intracellular ROS, milk supplemented with fresh ML does not induce significant oxidative stress. However, when stored for 4 hours at room temperature, ML induces significant levels of oxidative stress. Decreases in TEER and increases in apoptosis and IL-6 secretion are consistent with ML-induced oxidative stress. It therefore is likely that in clinical situations, if ML-supplemented milk is not administered quickly, the newborn may be placed at greater risk of oxidative stress.
- Subjects :
- Analysis of Variance
Apoptosis drug effects
Biological Assay
Caco-2 Cells
Food Preservation methods
Humans
Hydrogen Peroxide analysis
Interleukin-6 metabolism
Lipid Peroxidation drug effects
Temperature
Thiobarbituric Acid Reactive Substances analysis
Time Factors
Enterocytes metabolism
Fatty Acids pharmacology
Milk, Human immunology
Oxidative Stress drug effects
Reactive Oxygen Species analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1556-8342
- Volume :
- 2
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 18081458
- Full Text :
- https://doi.org/10.1089/bfm.2007.0017