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New insights into the BzdR-mediated transcriptional regulation of the anaerobic catabolism of benzoate in Azoarcus sp. CIB.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2008 Jan; Vol. 154 (Pt 1), pp. 306-316. - Publication Year :
- 2008
-
Abstract
- The expression of the bzd genes involved in the anaerobic degradation of benzoate in Azoarcus sp. CIB is controlled by the specific BzdR transcriptional repressor at the P(N) promoter. This catabolic promoter is also subject to catabolite repression by some organic acids. In vivo and in vitro experiments have shown that BzdR behaves as a repressor of the P(R) promoter by overlapping the transcription initiation site as well as the -35 and -10 boxes, benzoyl-CoA being the inducer molecule. In addition, by using a P(N) : : lacZ fusion both in Azoarcus sp. CIB and in an isogenic strain lacking the bzdR gene, we have shown that the succinate-dependent catabolite repression requires participation of the BzdR repressor.
- Subjects :
- Anaerobiosis
Artificial Gene Fusion
Base Sequence
Gene Deletion
Genes, Reporter
Molecular Sequence Data
Mutagenesis, Insertional
Promoter Regions, Genetic
Repressor Proteins genetics
beta-Galactosidase genetics
beta-Galactosidase metabolism
Azoarcus genetics
Azoarcus metabolism
Benzoates metabolism
Gene Expression Regulation, Bacterial
Repressor Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1350-0872
- Volume :
- 154
- Issue :
- Pt 1
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 18174149
- Full Text :
- https://doi.org/10.1099/mic.0.2007/011361-0