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Colorectal carcinomas from Middle East. Molecular and tissue microarray analysis of genomic instability pathways.
- Source :
-
Saudi medical journal [Saudi Med J] 2008 Jan; Vol. 29 (1), pp. 75-80. - Publication Year :
- 2008
-
Abstract
- Objective: To evaluate the overall incidence of microsatellite instability (MSI), hereditary non polyposis colorectal cancer, and tumor supressor gene (TP53) mutations in Saudi colorectal carcinomas.<br />Methods: We studied the MSI pathway in Saudi colorectal cancers (CRC) from 179 unselected patients using 2 methods: MSI by polymerase chain reaction, and immunohistochemistry detection of mutL homologs 1 and mutS homologs 2 proteins. The TP53 mutations were studied by sequencing exons 5, 6, 7, and 8.<br />Results: Of the 150 colorectal carcinomas analyzed for MSI, 16% of the tumors showed high level instability (MSI-H), 19.3% had low-level instability (MSI-L) and the remaining 64% tumors were stable. Survival of the MSI-H group was better as compared to the MSI-L or microsatellite stable group (p=0.0217). In the MSI-H group, 48% were familial MSI tumors, which could be attributable to the high incidence of consanguinity in the Saudi population. The TP53 mutations were found in 24% of the cases studied.<br />Conclusion: A high proportion of familial MSI cases and a lower incidence of TP53 mutations are some of the hallmarks of the Saudi colorectal carcinomas, which need to be explored further.
- Subjects :
- Chi-Square Distribution
Colorectal Neoplasms, Hereditary Nonpolyposis epidemiology
Colorectal Neoplasms, Hereditary Nonpolyposis ethnology
Genetic Markers
Genetics, Population
Humans
Immunohistochemistry
Incidence
Microarray Analysis
Mutation
Pilot Projects
Polymerase Chain Reaction
Saudi Arabia epidemiology
Colorectal Neoplasms, Hereditary Nonpolyposis genetics
Genes, p53 genetics
Microsatellite Instability
Subjects
Details
- Language :
- English
- ISSN :
- 0379-5284
- Volume :
- 29
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Saudi medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 18176677