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Triclosan-loaded poloxamine micelles for enhanced topical antibacterial activity against biofilm.
- Source :
-
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2008 Jun; Vol. 69 (2), pp. 535-45. Date of Electronic Publication: 2007 Dec 15. - Publication Year :
- 2008
-
Abstract
- Our research group is interested in the study of different technological approaches to treat hospital biofilm as a means to constrain nosocomial-acquired infections. The present work investigated the effect of the incorporation of the antibacterial agent triclosan (TS) into polymeric micelles of poloxamine T1107 (MW=15 kDa, 70 wt% PEO). The aggregation phenomenon was primarily investigated by means of Critical Micellar Concentration in a broad range of pH. Then, the effect of the polymer concentration on the micellar size was evaluated by Dynamic Light Scattering. Solubility levels increased up to 4 orders of magnitude. The drug inclusion affected the micellization, resulting in size increase and micellar fusion. This phenomenon was only apparent in TS-saturated systems. TS-loaded aggregates proved to be active in vitro against a broad spectrum of bacteria but more importantly, also against two representative clinical pathogens: methicilin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF). While the former was sensitive to even very low TS levels attainable in poloxamine-free aqueous media, the later was inhibited only when exposed to higher drug levels affordable exclusively using an inclusion system. These findings indicated the release of the drug from the reservoir. Finally, the activity of a TS-containing 5% poloxamine combination of pH 7.4 was assessed on biofilms of Staphylococcus epidermidis. Results showed a significant decrease (p<0.001) in the number of Colony-Formation Units when the biofilm was exposed to the TS/poloxamine as compared to the limited activity of the polymer-free TS control.
- Subjects :
- Anti-Infective Agents, Local chemistry
Cross Infection microbiology
Drug Stability
Enterococcus faecalis drug effects
Humans
Hydrogen-Ion Concentration
Light
Methicillin Resistance
Micelles
Poloxamer chemistry
Salmonella drug effects
Scattering, Radiation
Staphylococcus aureus drug effects
Staphylococcus epidermidis drug effects
Triclosan chemistry
Vancomycin Resistance
Anti-Infective Agents, Local administration & dosage
Anti-Infective Agents, Local pharmacology
Biofilms drug effects
Triclosan administration & dosage
Triclosan pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0939-6411
- Volume :
- 69
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
- Publication Type :
- Academic Journal
- Accession number :
- 18194853
- Full Text :
- https://doi.org/10.1016/j.ejpb.2007.11.021