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Antiproliferative effect of panaxynol on RASMCs via inhibition of ERK1/2 and CREB.
- Source :
-
Chemico-biological interactions [Chem Biol Interact] 2008 Feb 15; Vol. 171 (3), pp. 348-54. Date of Electronic Publication: 2007 Dec 08. - Publication Year :
- 2008
-
Abstract
- Panaxynol (PNN) occurs in many foods such as carrot, celery, and several reports have shown that it has neuritogenic and neuroprotective properties. In this study, we have investigated the antiproliferative effect and the mechanism of PNN on platelet-derived growth factor (PDGF)-BB-induced proliferation of rat aortic vascular smooth muscle cells (RASMCs). PNN significantly inhibited PDGF-BB-induced proliferation and DNA synthesis of RASMCs in a concentration-dependent manner. Flow cytometry analysis showed that PNN blocked the cell cycle progression at the G(1)/S phase. Preincubation of RASMCs with 9 microM PNN resulted in a significant inhibition of PDGF-BB-induced extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylation expression and PDGF-BB-induced CREB phosphorylation expression. The results indicated that the inhibitory effect of PNN on the PDGF-BB-induced proliferation of RASMCs might be mediated by blocking phosphorylation of ERK1/2 and that of CREB.
- Subjects :
- Animals
Aorta cytology
CREB-Binding Protein biosynthesis
Cell Cycle drug effects
Cells, Cultured
DNA biosynthesis
DNA drug effects
Diynes chemistry
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Fatty Alcohols chemistry
Mitogen-Activated Protein Kinase 1 biosynthesis
Mitogen-Activated Protein Kinase 3 biosynthesis
Molecular Conformation
Muscle, Smooth, Vascular cytology
Muscle, Smooth, Vascular metabolism
Phosphorylation drug effects
Rats
Signal Transduction drug effects
Time Factors
CREB-Binding Protein antagonists & inhibitors
Cell Proliferation drug effects
Diynes pharmacology
Fatty Alcohols pharmacology
Mitogen-Activated Protein Kinase 1 antagonists & inhibitors
Mitogen-Activated Protein Kinase 3 antagonists & inhibitors
Muscle, Smooth, Vascular drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0009-2797
- Volume :
- 171
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Chemico-biological interactions
- Publication Type :
- Academic Journal
- Accession number :
- 18199429
- Full Text :
- https://doi.org/10.1016/j.cbi.2007.11.013