Back to Search
Start Over
GBF-dependent family genes morphologically suppress the partially active Dictyostelium STATa strain.
- Source :
-
Development genes and evolution [Dev Genes Evol] 2008 Feb; Vol. 218 (2), pp. 55-68. Date of Electronic Publication: 2008 Jan 18. - Publication Year :
- 2008
-
Abstract
- Transcription factor Dd-STATa, a functional Dictyostelium homologue of metazoan signal transducers and activators of transcription proteins, is necessary for culmination during development. We have isolated more than 18 putative multicopy suppressors of Dd-STATa using genetic screening. One was hssA gene, whose expression is known to be G-box-binding-factor-dependent and which was specific to prestalk A (pstA) cells, where Dd-STATa is activated. Also, hssA mRNA was expressed in pstA cells in the Dd-STATa-null mutant. At least 40 hssA-related genes are present in the genome and constitute a multigene family. The tagged HssA protein was translated; hssA encodes an unusually high-glycine-serine-rich small protein (8.37 kDa), which has strong homology to previously reported cyclic-adenosine-monophosphate-inducible 2C and 7E proteins. Overexpression of hssA mRNA as well as frame-shifted versions of hssA RNA suppressed the phenotype of the partially active Dd-STATa strain, suggesting that translation is not necessary for suppression. Although overexpression of prespore-specific genes among the family did not suppress the parental phenotype, prestalk-specific family members did. Although overexpression of the hssA did not revert the expression of Dd-STATa target genes, and although its suppression mechanism remains unknown, morphological reversion implies functional relationships between Dd-STATa and hssA.
- Subjects :
- Animals
Dictyostelium classification
G-Box Binding Factors genetics
Gene Expression Regulation
Models, Genetic
Phylogeny
Protozoan Proteins metabolism
STAT Transcription Factors metabolism
Dictyostelium genetics
Genes, Protozoan
Genes, Suppressor
Protozoan Proteins genetics
STAT Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0949-944X
- Volume :
- 218
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Development genes and evolution
- Publication Type :
- Academic Journal
- Accession number :
- 18204858
- Full Text :
- https://doi.org/10.1007/s00427-008-0202-7