Back to Search
Start Over
The antibacterial efficacy of an aceraceous plant [Shantung maple (Acer truncatum Bunge)] may be related to inhibition of bacterial beta-oxoacyl-acyl carrier protein reductase (FabG).
- Source :
-
Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2008 Oct; Vol. 51 (Pt 2), pp. 73-8. - Publication Year :
- 2008
-
Abstract
- Polyphenols, including flavonoids, are the major components of the extracts from aceraceous plants. They possess remarkable antibacterial and antitumour activity. Our study focused on whether the inhibition of the bacterial type II fatty acid synthesis system is the mechanism for the antibacterial effect of the related plant polyphenols. Extracts obtained from the fallen leaves of the Shantung maple (Acer truncatum Bunge) using different solvents, and the related pure compound PGG (1,2,3,4,6-penta-O-galloyl-beta-D-glucose), potently inhibited the FabG (beta-oxoacyl-ACP reductase) steps in the fatty-acid-elongation cycle with the IC(50) values between 0.9 and 7.2 microg/ml. An ethyl acetate extract appeared to inhibit FabG reductase in a mixed manner with NADPH, as did PGG with NADPH, demonstrating that they interfered with the binding of the cofactor to the enzyme. Gram-positive and Gram-negative bacteria and some fungi were used to evaluate the antibacterial abilities of different extract samples. The experiments showed that a higher polyphenol content of the extracts led to a more potent inhibitory capacity against FabG, thus enhancing the antibacterial efficacy.
- Subjects :
- Alcohol Oxidoreductases chemistry
Alcohol Oxidoreductases isolation & purification
Anti-Bacterial Agents chemistry
Anti-Bacterial Agents isolation & purification
Binding Sites
Dose-Response Relationship, Drug
Drugs, Chinese Herbal chemistry
Drugs, Chinese Herbal isolation & purification
Enzyme Activation drug effects
Enzyme Inhibitors chemistry
Enzyme Inhibitors isolation & purification
Escherichia coli drug effects
Fungi drug effects
Gram-Negative Bacteria drug effects
Gram-Positive Bacteria drug effects
Inhibitory Concentration 50
Kinetics
Microbial Sensitivity Tests
Plant Leaves chemistry
Species Specificity
Structure-Activity Relationship
Time Factors
Acer chemistry
Alcohol Oxidoreductases antagonists & inhibitors
Anti-Bacterial Agents pharmacology
Drugs, Chinese Herbal pharmacology
Enzyme Inhibitors pharmacology
Escherichia coli enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1470-8744
- Volume :
- 51
- Issue :
- Pt 2
- Database :
- MEDLINE
- Journal :
- Biotechnology and applied biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 18208374
- Full Text :
- https://doi.org/10.1042/BA20070255