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Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon.
- Source :
-
BMC microbiology [BMC Microbiol] 2008 Jan 28; Vol. 8, pp. 20. Date of Electronic Publication: 2008 Jan 28. - Publication Year :
- 2008
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Abstract
- Background: The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. The general stress response, controlled by the alternative sigma factor, sigmaB, has an important role for bacterial survival both in the environment and during infection. We used quantitative real-time PCR analysis and immuno-blot analysis to examine sigmaB expression during growth of L. monocytogenes EGD-e. Whole genome-based transcriptional profiling was used to identify sigmaB-dependent genes at different growth phases.<br />Results: We detected 105 sigmaB-positively regulated genes and 111 genes which appeared to be under negative control of sigmaB and validated 36 sigmaB-positively regulated genes in vivo using a reporter gene fusion system.<br />Conclusion: Genes comprising the sigmaB regulon encode solute transporters, novel cell-wall proteins, universal stress proteins, transcriptional regulators and include those involved in osmoregulation, carbon metabolism, ribosome- and envelope-function, as well as virulence and niche-specific survival genes such as those involved in bile resistance and exclusion. Ten of the sigmaB-positively regulated genes of L. monocytogenes are absent in L. innocua. A total of 75 sigmaB-positively regulated listerial genes had homologs in B. subtilis, but only 33 have been previously described as being sigmaB-regulated in B. subtilis even though both species share a highly conserved sigmaB-dependent consensus sequence. A low overlap of genes may reflects adaptation of these bacteria to their respective environmental conditions.
- Subjects :
- Bile Acids and Salts pharmacology
Binding Sites
Biological Transport genetics
Chromosomes, Bacterial genetics
Energy Metabolism genetics
Gene Expression Regulation, Bacterial drug effects
Gene Expression Regulation, Developmental drug effects
Genomics methods
Immunoblotting
Listeria monocytogenes growth & development
Listeria monocytogenes metabolism
Oligonucleotide Array Sequence Analysis methods
Osmolar Concentration
Sigma Factor metabolism
Sigma Factor physiology
Transcription, Genetic drug effects
Gene Expression Profiling methods
Listeria monocytogenes genetics
Regulon genetics
Sigma Factor genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2180
- Volume :
- 8
- Database :
- MEDLINE
- Journal :
- BMC microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 18226246
- Full Text :
- https://doi.org/10.1186/1471-2180-8-20