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The human response to infection is associated with distinct patterns of interleukin 23 and interleukin 27 expression.

Authors :
O'Dwyer MJ
Mankan AK
White M
Lawless MW
Stordeur P
O'Connell B
Kelleher DP
McManus R
Ryan T
Source :
Intensive care medicine [Intensive Care Med] 2008 Apr; Vol. 34 (4), pp. 683-91. Date of Electronic Publication: 2008 Jan 29.
Publication Year :
2008

Abstract

Objective: The development and progression of severe sepsis is related to a deficiency in pro-inflammatory cytokine production, characterised by lesser IFNgamma levels, which are not explained by variations in levels of the main putative regulator of IFNgamma, namely IL-12. As alternative regulators of IFNgamma may be of greater importance in human sepsis, we investigated the hypothesis that the development of severe sepsis is related to variations in IL-18, IL-23 and IL-27 gene expression.<br />Design and Setting: A prospective observational trial in a mixed intensive care unit (ICU) and hospital wards in a university teaching hospital.<br />Patients and Participants: Sixty-two ICU patients with severe sepsis, 13 bacteraemic patients with no acute critical illness, and 10 healthy controls.<br />Measurements and Results: All subjects were assayed for IL-18, IL-23 and IL-27 mRNA levels in peripheral blood. IL-27 mRNA levels distinguished between the three groups, with levels highest in the ICU group, intermediate in the bacteraemic group and lowest in the control group. IL-23 distinguished between the groups, with levels lowest in the ICU group. In late sepsis IL-23 and TNFalpha mRNA levels were directly related. IL-18 mRNA levels did not distinguish between the patient groups.<br />Conclusions: We conclude that the deficient pro-inflammatory response in patients with sepsis is expansive and includes deficient IL-23 and excessive IL-27 gene expression. This provides further evidence that upregulation of a cytokine-based immune response is beneficial in sepsis.

Details

Language :
English
ISSN :
0342-4642
Volume :
34
Issue :
4
Database :
MEDLINE
Journal :
Intensive care medicine
Publication Type :
Academic Journal
Accession number :
18227999
Full Text :
https://doi.org/10.1007/s00134-007-0968-5