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The human response to infection is associated with distinct patterns of interleukin 23 and interleukin 27 expression.
- Source :
-
Intensive care medicine [Intensive Care Med] 2008 Apr; Vol. 34 (4), pp. 683-91. Date of Electronic Publication: 2008 Jan 29. - Publication Year :
- 2008
-
Abstract
- Objective: The development and progression of severe sepsis is related to a deficiency in pro-inflammatory cytokine production, characterised by lesser IFNgamma levels, which are not explained by variations in levels of the main putative regulator of IFNgamma, namely IL-12. As alternative regulators of IFNgamma may be of greater importance in human sepsis, we investigated the hypothesis that the development of severe sepsis is related to variations in IL-18, IL-23 and IL-27 gene expression.<br />Design and Setting: A prospective observational trial in a mixed intensive care unit (ICU) and hospital wards in a university teaching hospital.<br />Patients and Participants: Sixty-two ICU patients with severe sepsis, 13 bacteraemic patients with no acute critical illness, and 10 healthy controls.<br />Measurements and Results: All subjects were assayed for IL-18, IL-23 and IL-27 mRNA levels in peripheral blood. IL-27 mRNA levels distinguished between the three groups, with levels highest in the ICU group, intermediate in the bacteraemic group and lowest in the control group. IL-23 distinguished between the groups, with levels lowest in the ICU group. In late sepsis IL-23 and TNFalpha mRNA levels were directly related. IL-18 mRNA levels did not distinguish between the patient groups.<br />Conclusions: We conclude that the deficient pro-inflammatory response in patients with sepsis is expansive and includes deficient IL-23 and excessive IL-27 gene expression. This provides further evidence that upregulation of a cytokine-based immune response is beneficial in sepsis.
- Subjects :
- Adult
Aged
Aged, 80 and over
Bacteremia immunology
Case-Control Studies
Disease Progression
Female
Gene Expression Regulation
Humans
Interferon-gamma genetics
Interferon-gamma immunology
Interleukin-17 genetics
Interleukin-18 genetics
Interleukin-23 genetics
Male
Middle Aged
Prospective Studies
RNA, Messenger blood
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Interferon-gamma deficiency
Interleukin-17 blood
Interleukin-18 blood
Interleukin-23 blood
Systemic Inflammatory Response Syndrome immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0342-4642
- Volume :
- 34
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Intensive care medicine
- Publication Type :
- Academic Journal
- Accession number :
- 18227999
- Full Text :
- https://doi.org/10.1007/s00134-007-0968-5