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[Analysis of pde6b mRNA sequences of Kunming mice and C57BL/6J mice].

Authors :
Yan GL
Wu YS
Guo Q
An J
Liu XP
Zhang ZM
Source :
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2008 Feb; Vol. 24 (2), pp. 130-2.
Publication Year :
2008

Abstract

Aim: To clone and sequence the phosphodiesterase 6 beta subunit (pde6b) gene of Kunming mice, and to compare it with counterpart sequences in the GenBank database.<br />Methods: The primers were designed covering the CDS region of the pde6b gene, and the corresponding fragments were amplified using RT-PCR. The fragments were cloned into plasmids, amplified in E.coli, and sequenced. Bioinformatics programs and online tools were used to analyze the sequences.<br />Results: The CDS of the pde6b gene of Kunming mice was cloned and sequenced. Compared with the inbred mice C57BL/6J, pde6b CDS sequence of Kunming mice was different in some points: a G-->A transition at +706, a C-->T transversion at +1149. The protein sequence was of little difference: only a glycine-->serine at +236.<br />Conclusion: The pde6b CDS region of Kunming mice was successfully cloned. Pde6b sequences are of some level difference in different kinds of mice, but they are most conserved in protein level.

Details

Language :
Chinese
ISSN :
1007-8738
Volume :
24
Issue :
2
Database :
MEDLINE
Journal :
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Publication Type :
Academic Journal
Accession number :
18237528