Back to Search
Start Over
[Study of the effect of hepatitis C virus core protein on interferon-induced antiviral genes expression and its mechanisms].
- Source :
-
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2007 Nov; Vol. 23 (6), pp. 1000-4. - Publication Year :
- 2007
-
Abstract
- To study the effect of HCV core protein on the interferon-induced antiviral genes expression and its mechanisms. Methods HepG2 cells were transiently transfected with HCV core protein expression plasmid and the blank plasmid respectively. RT-PCR was used to analyze the effect of HCV core protein on PKR and 2'-5'OAS expression. The effect of HCV core protein on ISRE-medicated gene expression was detected by luciferase activity assay. Western-blot assay was performed to observe the change of mRNA and protein levels of SOCS3, STAT1 and p-STAT1 following HCV core expression. In the presence of HCV core protein, the transcription of PKR and 2'-5' OAS are down-regulated. ISRE-medicated reporter gene expression and STAT1 phosphorylation were inhibited. The transcription and expression of SOCS3 were induced compared with blank plasmid-transfected group. In HepG2 cells, HCV core protein can down-regulate the expression of some interferon-induced antiviral genes, which involves the induction of SOCS3 and the inhibition of STAT1 phosphorylation.
- Subjects :
- 2',5'-Oligoadenylate Synthetase genetics
2',5'-Oligoadenylate Synthetase metabolism
Carcinoma, Hepatocellular pathology
Down-Regulation
Hepacivirus metabolism
Humans
Interferon-Stimulated Gene Factor 3 genetics
Interferon-alpha genetics
Liver Neoplasms pathology
Protein Kinases genetics
Protein Kinases metabolism
STAT1 Transcription Factor genetics
STAT1 Transcription Factor metabolism
STAT2 Transcription Factor genetics
STAT2 Transcription Factor metabolism
Transfection
Tumor Cells, Cultured
Viral Core Proteins genetics
Viral Core Proteins metabolism
Hepacivirus genetics
Interferon-Stimulated Gene Factor 3 metabolism
Interferon-alpha immunology
Transcription, Genetic
Viral Core Proteins physiology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-3061
- Volume :
- 23
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 18257226