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Ecto-nucleotidase pathway is altered by different treatments with fluoxetine and nortriptyline.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2008 Mar 31; Vol. 583 (1), pp. 18-25. Date of Electronic Publication: 2008 Jan 26. - Publication Year :
- 2008
-
Abstract
- Depression is one of the most disabling diseases and causes a significant burden to both individual and society. Selective serotonin reuptake inhibitors and tricyclic antidepressants, such as fluoxetine and nortriptyline, respectively, are commonly used in treatment for depression. These antidepressants were tested on cerebral cortex and hippocampal synaptosomes after acute and chronic in vivo and in vitro treatments. In chronic treatment, fluoxetine and nortriptyline decreased ATP hydrolysis (17.8% and 16.3%, respectively) in hippocampus. In cerebral cortex, nortriptyline increased ATP (32.3%), ADP (51.8%), and AMP (59.5%) hydrolysis. However, fluoxetine decreased ATP (25.5%) hydrolysis and increased ADP (80.1%) and AMP (33.3%) hydrolysis. Significant activation of ADP hydrolysis was also observed in acute treatment with nortriptyline (49.8%) in cerebral cortex. However, in hippocampus, ATP (24.7%) and ADP (46.1%) hydrolysis were inhibited. Fluoxetine did not alter enzyme activities in acute treatment for both structures. In addition, there were significant changes in NTPDase activities when fluoxetine and nortriptyline (100, 250, and 500 microM) were tested in vitro. There was no inhibitory effect of fluoxetine and nortriptyline on AMP hydrolysis in cerebral cortex and hippocampus. The findings showed that these antidepressant drugs can affect the ecto-nucleotidase pathway, suggesting that the extracellular adenosine levels could be modulated by these drugs.
- Subjects :
- Adenosine metabolism
Adenosine Triphosphate metabolism
Animals
Cerebral Cortex drug effects
Cerebral Cortex metabolism
Extracellular Space drug effects
Extracellular Space metabolism
Hippocampus drug effects
Hippocampus metabolism
Isoenzymes metabolism
Male
Norepinephrine metabolism
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Serotonin metabolism
Signal Transduction drug effects
Synaptosomes drug effects
Synaptosomes metabolism
Adenosine Triphosphatases metabolism
Antidepressive Agents, Second-Generation pharmacology
Antidepressive Agents, Tricyclic pharmacology
Fluoxetine pharmacology
Nortriptyline pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2999
- Volume :
- 583
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 18280468
- Full Text :
- https://doi.org/10.1016/j.ejphar.2008.01.013