Back to Search
Start Over
HPLC-ESI-MS/MS validated method for simultaneous quantification of zopiclone and its metabolites, N-desmethyl zopiclone and zopiclone-N-oxide in human plasma.
- Source :
-
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2008 Mar 15; Vol. 864 (1-2), pp. 137-48. Date of Electronic Publication: 2008 Feb 17. - Publication Year :
- 2008
-
Abstract
- A simple, selective and sensitive isocratic HPLC method with triple quadrupole mass spectrometry detection has been developed and validated for simultaneous quantification of zopiclone and its metabolites in human plasma. The analytes were extracted using solid phase extraction, separated on Symmetry shield RP8 column (150 mm x 4.6 mm i.d., 3.5 microm particle size) and detected by tandem mass spectrometry with a turbo ion spray interface. Metaxalone was used as an internal standard. The method had a chromatographic run time of 4.5 min and linear calibration curves over the concentration range of 0.5-150 ng/mL for both zopiclone and N-desmethyl zopiclone and 1-150 ng/mL for zopiclone-N-oxide. The intra-batch and inter-batch accuracy and precision evaluated at lower limit of quantification and quality control levels were within 89.5-109.1% and 3.0-14.7%, respectively, for all the analytes. The recoveries calculated for the analytes and internal standard were > or = 90% from spiked plasma samples. The validated method was successfully employed for a comparative bioavailability study after oral administration of 7.5 mg zopiclone (test and reference) to 16 healthy volunteers under fasted condition.
- Subjects :
- Azabicyclo Compounds administration & dosage
Azabicyclo Compounds pharmacokinetics
Biological Availability
Drug Stability
Fasting
Humans
Piperazines administration & dosage
Piperazines pharmacokinetics
Quality Control
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization methods
Tandem Mass Spectrometry methods
Azabicyclo Compounds blood
Chromatography, High Pressure Liquid methods
Hypnotics and Sedatives blood
Mass Spectrometry methods
Piperazines blood
Subjects
Details
- Language :
- English
- ISSN :
- 1570-0232
- Volume :
- 864
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 18313371
- Full Text :
- https://doi.org/10.1016/j.jchromb.2008.02.004