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[Role of connective tissue growth factor on pulmonary artery remodeling in rats exposed to smoke].
- Source :
-
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases [Zhonghua Jie He He Hu Xi Za Zhi] 2007 Dec; Vol. 30 (12), pp. 921-5. - Publication Year :
- 2007
-
Abstract
- Objective: To explore the role of connective tissue growth factor (CTGF) on pulmonary artery remodeling induced by smoke exposure in rats.<br />Methods: Thirty-five male Wistar rats were randomly assigned into a control group (A group), a smoke exposure one month group (B group), a smoke exposure and high dose CTGF antisense oligonucleotide (ASON) one month group (C group), a smoke exposure and low dose CTGF ASON one month group (D group), a smoke exposure two month group (E group), a smoke exposure and high dose CTGF ASON two month group (F group), and a smoke exposure and low dose CTGF ASON two month group (G group). Pulmonary artery remodeling was observed by hematoxylin-eosin staining, and the CTGF mRNA expressions of pulmonary arteries were evaluated by RT-PCR. Immunohistochemistry methods were performed to determine CTGF protein expression in pulmonary artery smooth muscle. The difference between the groups was analyzed.<br />Results: (1) The pulmonary artery WA% of the seven groups were respectively (28.6 +/- 1.2)%, (42.5 +/- 2.3)%, (33.7 +/- 1.8)%, (42.1 +/- 2.4)%, (49.6 +/- 2.1)%, (34.3 +/- 1.9)% and (38.4 +/- 2.0)%. There was significant difference between B group and C group (q = 5.09, P < 0.01). Compared to E group, there were significant decreases in F group and G group (q = 8.15, 3.75, all P < 0.05). (2) The CTGF protein expressions (A value) of pulmonary artery smooth muscle were respectively 0.098 +/- 0.015, 0.159 +/- 0.023, 0.118 +/- 0.017, 0.153 +/- 0.022, 0.406 +/- 0.036, 0.109 +/- 0.012 and 0.146 +/- 0.024. There was significant difference between B group and C group (q = 3.26, P < 0.05). Compared to E group, there were significant decreases in F group and G group (q = 67.08, 18.09, all P < 0.01). (3) The CTGF mRNA expressions (A(CTGF)/A(beta-actin)) of pulmonary artery were respectively 0.051 +/- 0.010, 0.823 +/- 0.096, 0.216 +/- 0.056, 0.810 +/- 0.085, 2.452 +/- 0.267, 0.207 +/- 0.062 and 0.509 +/- 0.067. There was significant difference between B group and C group (q = 53.50, P < 0.01). Compared to E group, there were significant decreases in F group and G group (q = 132.22, 93.70, all P < 0.01).<br />Conclusion: Administration of CTGF ASON in vivo could significantly reduce pulmonary artery remodeling induced by smoke exposure in rats, indicating that CTGF may play an important role in pulmonary hypertension.
- Subjects :
- Animals
Connective Tissue Growth Factor genetics
Connective Tissue Growth Factor metabolism
Immunohistochemistry
Lung drug effects
Lung metabolism
Lung pathology
Male
Muscle, Smooth, Vascular drug effects
Muscle, Smooth, Vascular metabolism
Muscle, Smooth, Vascular pathology
Oligodeoxyribonucleotides, Antisense administration & dosage
Pulmonary Artery metabolism
Pulmonary Artery physiopathology
RNA, Messenger genetics
RNA, Messenger metabolism
Random Allocation
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Nicotiana chemistry
Connective Tissue Growth Factor physiology
Oligodeoxyribonucleotides, Antisense pharmacology
Pulmonary Artery drug effects
Smoke
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-0939
- Volume :
- 30
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
- Publication Type :
- Academic Journal
- Accession number :
- 18336769