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Alteration of glyoxalase genes expression in response to testosterone in LNCaP and PC3 human prostate cancer cells.
- Source :
-
Cancer biology & therapy [Cancer Biol Ther] 2007 Dec; Vol. 6 (12), pp. 1880-8. Date of Electronic Publication: 2007 Sep 01. - Publication Year :
- 2007
-
Abstract
- Glyoxalase system, a ubiquitous detoxification pathway protecting against cellular damage caused by potent cytotoxic metabolites, is involved in the regulation of cellular growth. Aberrations in the expression of glyoxalase genes in several human cancers have been reported. Recently, we described a possible regulatory effect by estrogens on glyoxalase genes in human breast cancer cell lines. This result, along with those ones regarding changes in glyoxalases activity and expression in other human hormone-regulated cancers, such as prostate cancer, has prompted us to investigate whether also androgens, whose functional role in prostate cancer pathogenesis is well known, could modulate glyoxalases gene expression. Therefore, we treated LNCaP androgen-responsive and PC3 androgen-independent human prostate cancer cell lines with testosterone at the concentrations of 1 nM and 100 nM. After a two days treatment, glyoxalases mRNA levels as well as cell proliferation were evaluated by real-time RT-PCR analysis and [3H]thymidine incorporation, respectively. Results pointed out that testosterone affects the expression of glyoxalase system genes and cell proliferation in a different manner in the two cell lines. The possibility that modulation of glyoxalase genes expression by testosterone is due to glyoxalases-mediated intracellular response mechanisms to the androgen-induced oxidative stress or to the presence of androgen response elements (ARE) in glyoxalase promoters are discussed. Knowledge regarding the regulation of glyoxalases by testosterone may provide insights into the importance of these enzymes in human prostate carcinomas in vivo.
- Subjects :
- Adenocarcinoma enzymology
Aromatase Inhibitors pharmacology
Base Sequence
Cell Line, Tumor drug effects
Cell Line, Tumor enzymology
Enzyme Induction drug effects
Estradiol pharmacology
Humans
Lactoylglutathione Lyase biosynthesis
Letrozole
Male
Molecular Sequence Data
Neoplasm Proteins biosynthesis
Neoplasms, Hormone-Dependent enzymology
Nitriles pharmacology
Prostatic Neoplasms enzymology
Prostatic Neoplasms pathology
Pyruvaldehyde metabolism
RNA, Messenger biosynthesis
RNA, Neoplasm biosynthesis
Response Elements genetics
Thiolester Hydrolases biosynthesis
Triazoles pharmacology
Adenocarcinoma pathology
Androgens
Gene Expression Regulation, Neoplastic drug effects
Lactoylglutathione Lyase genetics
Neoplasm Proteins genetics
Neoplasms, Hormone-Dependent pathology
Testosterone pharmacology
Thiolester Hydrolases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1555-8576
- Volume :
- 6
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cancer biology & therapy
- Publication Type :
- Academic Journal
- Accession number :
- 18344682
- Full Text :
- https://doi.org/10.4161/cbt.6.12.4961