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Hexavalent molybdenum reduction to molybdenum blue by S. marcescens strain Dr. Y6.
- Source :
-
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2008 Apr; Vol. 149 (1), pp. 33-43. Date of Electronic Publication: 2008 Feb 20. - Publication Year :
- 2008
-
Abstract
- A molybdate-reducing bacterium has been locally isolated. The bacterium reduces molybdate or Mo(6+) to molybdenum blue (molybdate oxidation states of between 5+ and 6+). Different carbon sources such as acetate, formate, glycerol, citric acid, lactose, fructose, glucose, mannitol, tartarate, maltose, sucrose, and starch were used at an initial concentration of 0.2% (w/v) in low phosphate media to study their effect on the molybdate reduction efficiency of bacterium. All of the carbon sources supported cellular growth, but only sucrose, maltose, glucose, and glycerol (in decreasing order) supported molybdate reduction after 24 h of incubation. Optimum concentration of sucrose for molybdate reduction is 1.0% (w/v) after 24 h of static incubation. Ammonium sulfate, ammonium chloride, valine, OH-proline, glutamic acid, and alanine (in the order of decreasing efficiency) supported molybdate reduction with ammonium sulfate giving the highest amount of molybdenum blue after 24 h of incubation at 0.3% (w/v). The optimum molybdate concentration that supports molybdate reduction is between 15 and 25 mM. Molybdate reduction is optimum at 35 degrees C. Phosphate at concentrations higher than 5 mM strongly inhibits molybdate reduction. The molybdenum blue produced from cellular reduction exhibits a unique absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. The isolate was tentatively identified as Serratia marcescens Strain Dr.Y6 based on carbon utilization profiles using Biolog GN plates and partial 16s rDNA molecular phylogeny.
- Subjects :
- Carbon pharmacology
Hydrogen-Ion Concentration drug effects
Molybdenum pharmacology
Nitrogen pharmacology
Oxidation-Reduction drug effects
Phosphates pharmacology
Phylogeny
Serratia marcescens cytology
Serratia marcescens drug effects
Serratia marcescens isolation & purification
Spectrum Analysis
Temperature
Molybdenum metabolism
Serratia marcescens metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0273-2289
- Volume :
- 149
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Applied biochemistry and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 18350385
- Full Text :
- https://doi.org/10.1007/s12010-008-8137-z