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[Tissue distribution and excretion of 5-fluorouracil from indomethacin 5-fluorouracil-1-ylmethylester in rats].
- Source :
-
Yao xue xue bao = Acta pharmaceutica Sinica [Yao Xue Xue Bao] 2008 Jan; Vol. 43 (1), pp. 81-5. - Publication Year :
- 2008
-
Abstract
- To study the tissue distribution and excretion of indomethacin 5-fluorouracil-1-ylmethyl ester (IFM) metabolite 5-fluorouracil in rats, an accurate and specific high performance liquid chromatography method for quantifying IFM in rat plasma and tissues was developed. Biological samples were prepared by liquid-liquid extraction and separated on a Diamonsil C18 column (250 mm x 4.6 mm ID, 5 microm). The mobile phase for tissue samples, plasma samples and feces samples were composed of methanol-water-36% acetic acid (3:96.9:0.1, v/v) and the mobile phase for urine samples was a mixture of methanol-water-36% acetic acid (10:89.9:0.1, v/v). The eluate was monitored by UV absorbance at 260 nm. After a single ig dose of 100 mg x kg(-1) IFM in rats, 5-Fu was mainly distributed in stomach, small intestine, and liver. The concentrations of 5-fluorouracil in other tissues and plasma were low. The excretion of 5-Fu in urine and feces amounted to 0.0065% and 0.063% of the dose, respectively. The method is shown to be accurate and specific, and suitable for preclinical pharmacokinetic studies of IFM.
- Subjects :
- Animals
Anti-Inflammatory Agents, Non-Steroidal metabolism
Anti-Inflammatory Agents, Non-Steroidal pharmacokinetics
Anti-Inflammatory Agents, Non-Steroidal urine
Antimetabolites, Antineoplastic pharmacokinetics
Antimetabolites, Antineoplastic urine
Feces chemistry
Female
Fluorouracil urine
Indomethacin pharmacokinetics
Indomethacin urine
Male
Random Allocation
Rats
Rats, Wistar
Sensitivity and Specificity
Tissue Distribution
Fluorouracil pharmacokinetics
Indomethacin metabolism
Prodrugs pharmacokinetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0513-4870
- Volume :
- 43
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Yao xue xue bao = Acta pharmaceutica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 18357738