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[Role of Smac in cisplatin-induced apoptosis of non-small cell lung cancer cells].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2008 Mar; Vol. 28 (3), pp. 389-91. - Publication Year :
- 2008
-
Abstract
- Objective: To investigate the role of Smac in cisplatin-induced apoptosis of non-small lung cancer cells in vitro.<br />Methods: Non-small cell lung cancer A549 cells were incubated in the presence of cisplatin at different concentrations, and the cell proliferation status was observed using MTT assay. Flow cytometry was used for evaluation of the apoptosis of the incubated cells, and the expressions of Smac mRNA and protein were detected by RT-PCR and Western blotting, respectively.<br />Results: Cisplatin inhibited the proliferation and induced apoptosis of A549 cells both in a concentration-dependent manner. Cisplatin also increased the expression of Smac at both the mRNA and protein levels, which was also concentration-dependent.<br />Conclusion: Increased Smac expression may play a critical role in cisplatin-induced apoptosis of the non-small cell lung cancer cells in vitro.
- Subjects :
- Antineoplastic Agents pharmacology
Apoptosis Regulatory Proteins
Blotting, Western
Carcinoma, Non-Small-Cell Lung genetics
Carcinoma, Non-Small-Cell Lung metabolism
Carcinoma, Non-Small-Cell Lung pathology
Cell Line, Tumor
Dose-Response Relationship, Drug
Humans
Inhibitor of Apoptosis Proteins biosynthesis
Inhibitor of Apoptosis Proteins genetics
Intracellular Signaling Peptides and Proteins genetics
Lung Neoplasms genetics
Lung Neoplasms metabolism
Lung Neoplasms pathology
Mitochondrial Proteins genetics
RNA, Messenger biosynthesis
RNA, Messenger genetics
Reverse Transcriptase Polymerase Chain Reaction
Apoptosis drug effects
Cisplatin pharmacology
Mitochondrial Proteins biosynthesis
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 18359697