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Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid VP2 protein carrying immunoreactive epitope of porcine circovirus type 2.
- Source :
-
Vaccine [Vaccine] 2008 Apr 16; Vol. 26 (17), pp. 2119-26. Date of Electronic Publication: 2008 Mar 13. - Publication Year :
- 2008
-
Abstract
- Virus-like particles (VLPs) are non-replicative vectors for delivery of heterologous epitopes and induction of immune responses. In this study, a self-assembled porcine parvovirus (PPV) VP2 capsid protein [PPV:VLP-(PCV2)] carrying immunoreactive epitopes, residues 165-200 from the Porcine circovirus 2 (PCV2) virus nucleoprotein was constructed. Immunogenicity study was carried out with hybrid VLPs derived from HEK-293 cells infected with recombinant adenovirus vectors. To our knowledge, this study presents the first demonstration that hybrid non-replicative PPV VLPs carrying PCV2 immunoreactive epitopes can induce stronger antibody responses against PCV2 than recombinant adenovirus of PCV2 ORF2, in the absence of any adjuvant. The hybrid VLPs [PPV:VLP-(PCV2)] might be a promising candidate vaccine for better prevention of the diseases associated with PCV2 as well as with co-infection by PCV2 and PPV.
- Subjects :
- Animals
Capsid Proteins genetics
Cell Line
Circovirus genetics
DNA, Recombinant genetics
Epitopes genetics
Epitopes immunology
Mice
Parvovirus, Porcine genetics
Recombinant Proteins genetics
Recombinant Proteins immunology
Viral Vaccines genetics
Viral Vaccines immunology
Antigens, Viral immunology
Capsid Proteins immunology
Circovirus immunology
DNA, Recombinant immunology
Genetic Vectors chemistry
Parvovirus, Porcine immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 26
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 18378364
- Full Text :
- https://doi.org/10.1016/j.vaccine.2008.02.037