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Quantification of liver perfusion with [(15)O]H(2)O-PET and its relationship with glucose metabolism and substrate levels.
- Source :
-
Journal of hepatology [J Hepatol] 2008 Jun; Vol. 48 (6), pp. 974-82. Date of Electronic Publication: 2008 Mar 04. - Publication Year :
- 2008
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Abstract
- Background/aims: Hepatic perfusion plays an important role in liver physiology and disease. This study was undertaken to (a) validate the use of Positron Emission Tomography (PET) and oxygen-15-labeled water ([(15)O]H(2)O) to quantify hepatic and portal perfusion, and (b) examine relationships between portal perfusion and liver glucose and lipid metabolism.<br />Methods: Liver [(15)O]H(2)O-PET images were obtained in 14 pigs during fasting or hyperinsulinemia. Carotid arterial and portal venous blood were sampled for [(15)O]H(2)O activity; Doppler ultrasonography was used invasively as the reference method. A single arterial input compartment model was developed to estimate portal tracer kinetics and liver perfusion. Endogenous glucose production (EGP) and insulin-mediated whole body glucose uptake (wbGU) were determined by standard methods.<br />Results: Hepatic arterial and portal venous perfusions were 0.15+/-0.07 and 1.11+/-0.34 ml/min/ml of tissue, respectively. The agreement between ultrasonography and [(15)O]H(2)O-PET was good for total and portal liver perfusion, and poor for arterial perfusion. Portal perfusion was correlated with EGP (r=or+0.62, p=0.03), triglyceride (r=or+0.66, p=0.01), free fatty acid levels (r=or+0.76, p=0.003), and plasma lactate levels (r=or-0.81, p=0.0009).<br />Conclusions: Estimates of liver perfusion by [(15)O]H(2)O-PET compared well with those by ultrasonography. The method allowed to predict portal tracer concentrations which is essential in human studies. Portal perfusion may affect liver nutrient handling.
- Subjects :
- Animals
Fasting metabolism
Fatty Acids, Nonesterified metabolism
Hyperinsulinism metabolism
Lactates metabolism
Lipid Metabolism physiology
Liver diagnostic imaging
Models, Biological
Oxygen Radioisotopes
Swine
Triglycerides metabolism
Ultrasonography, Doppler
Glucose metabolism
Liver metabolism
Perfusion methods
Positron-Emission Tomography
Subjects
Details
- Language :
- English
- ISSN :
- 0168-8278
- Volume :
- 48
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 18384905
- Full Text :
- https://doi.org/10.1016/j.jhep.2008.01.029