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A simple restriction fragment PCR approach for discrimination of humanpathogenic Old World animal Orthopoxvirus species.

Authors :
Huemer HP
Hönlinger B
Höpfl R
Source :
Canadian journal of microbiology [Can J Microbiol] 2008 Feb; Vol. 54 (2), pp. 159-62.
Publication Year :
2008

Abstract

There are reliable polymerase chain reaction assays available for exclusion of Variola virus from other poxviruses. However, the discrimination of humanpathogenic animal Orthopoxviridae is more challenging because of the high genomic conservation. Based on the variability of the A36R gene, we describe a simple 20 min PCR assay followed by a 1 h digest with 3 different restriction enzymes. This assay enables rapid discrimination between Cowpox virus and Monkeypox virus and discrimination of the most prevalent members of the Vaccinia virus and Camelpox virus. The test was orthopoxvirus specificand did not react with parapox (Orf) virus. Moreover, the amplified fragments were also well suited for additional genotyping by direct DNA sequencing.

Details

Language :
English
ISSN :
0008-4166
Volume :
54
Issue :
2
Database :
MEDLINE
Journal :
Canadian journal of microbiology
Publication Type :
Academic Journal
Accession number :
18388986
Full Text :
https://doi.org/10.1139/w07-129