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Enumeration of functionally active anti-Aspergillus T-cells in human peripheral blood.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2008 Jun 01; Vol. 335 (1-2), pp. 41-5. Date of Electronic Publication: 2008 Mar 20. - Publication Year :
- 2008
-
Abstract
- Invasive aspergillosis remains a life-threatening complication in patients undergoing allogeneic stem cell transplantation (SCT). Since CD4(+) T-cells provide a critical secondary defense against Aspergillus spp., the quantification of "functional" anti-Aspergillus T-cells might be important in the clinical care of allogeneic transplant patients. We present a rapid, simple and reproducible method to enumerate functionally active, cytokine-producing anti-Aspergillus T-cells in peripheral blood by means of flow cytometry, by which these cells were also phenotypically characterized as memory CD4(+) T-cells. When using 100,000 PBMCs and requiring a minimum of 50 events, at least one anti-Aspergillus T-cell among 1000 CD4(+) T-cells can be detected. Compared to healthy individuals, the number of anti-Aspergillus T-cells in patients up to one year after SCT was significantly lower. The presented method might help to define hematopoietic transplant recipients who will benefit from adoptive transfer of anti-Aspergillus T cells.
- Subjects :
- Adolescent
Adult
Antigens, Fungal
Aspergillosis etiology
Aspergillosis microbiology
CD4-Positive T-Lymphocytes microbiology
Case-Control Studies
Cells, Cultured
Child
Female
Humans
Immunologic Memory
Male
Phenotype
Reproducibility of Results
Time Factors
Aspergillosis immunology
Aspergillus fumigatus immunology
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes immunology
Cytokines metabolism
Flow Cytometry
Hematopoietic Stem Cell Transplantation adverse effects
Immunophenotyping methods
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 335
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 18395221
- Full Text :
- https://doi.org/10.1016/j.jim.2008.02.014