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Enumeration of functionally active anti-Aspergillus T-cells in human peripheral blood.

Authors :
Beck O
Koehl U
Tramsen L
Mousset S
Latgé JP
Müller K
Schwabe D
Bader P
Klingebiel T
Lehrnbecher T
Source :
Journal of immunological methods [J Immunol Methods] 2008 Jun 01; Vol. 335 (1-2), pp. 41-5. Date of Electronic Publication: 2008 Mar 20.
Publication Year :
2008

Abstract

Invasive aspergillosis remains a life-threatening complication in patients undergoing allogeneic stem cell transplantation (SCT). Since CD4(+) T-cells provide a critical secondary defense against Aspergillus spp., the quantification of "functional" anti-Aspergillus T-cells might be important in the clinical care of allogeneic transplant patients. We present a rapid, simple and reproducible method to enumerate functionally active, cytokine-producing anti-Aspergillus T-cells in peripheral blood by means of flow cytometry, by which these cells were also phenotypically characterized as memory CD4(+) T-cells. When using 100,000 PBMCs and requiring a minimum of 50 events, at least one anti-Aspergillus T-cell among 1000 CD4(+) T-cells can be detected. Compared to healthy individuals, the number of anti-Aspergillus T-cells in patients up to one year after SCT was significantly lower. The presented method might help to define hematopoietic transplant recipients who will benefit from adoptive transfer of anti-Aspergillus T cells.

Details

Language :
English
ISSN :
0022-1759
Volume :
335
Issue :
1-2
Database :
MEDLINE
Journal :
Journal of immunological methods
Publication Type :
Academic Journal
Accession number :
18395221
Full Text :
https://doi.org/10.1016/j.jim.2008.02.014