Back to Search
Start Over
Activation of interleukin-32 pro-inflammatory pathway in response to influenza A virus infection.
- Source :
-
PloS one [PLoS One] 2008 Apr 16; Vol. 3 (4), pp. e1985. Date of Electronic Publication: 2008 Apr 16. - Publication Year :
- 2008
-
Abstract
- Background: Interleukin (IL)-32 is a recently described pro-inflammatory cytokine that has been reported to be induced by bacteria treatment in culture cells. Little is known about IL-32 production by exogenous pathogens infection in human individuals.<br />Methods and Findings: In this study, we found that IL-32 level was increased by 58.2% in the serum samples from a cohort of 108 patients infected by influenza A virus comparing to that of 115 healthy individuals. Another pro-inflammatory factor cyclooxygenase (COX)-2-associated prostaglandin E2 was also upregulated by 2.7-fold. Expression of IL-32 in influenza A virus infected A549 human lung epithelial cells was blocked by either selective COX-2 inhibitor NS398 or Aspirin, a known anti-inflammatory drug, indicating IL-32 was induced through COX-2 in the inflammatory cascade. Interestingly, we found that COX-2-associate PGE(2) production activated by influenza virus infection was significantly suppressed by over-expression of IL-32 but increased by IL-32-specific siRNA, suggesting there was a feedback mechanism between IL-32 and COX-2.<br />Conclusions: IL-32 is induced by influenza A virus infection via COX-2 in the inflammatory cascade. Our results provide that IL-32 is a potential target for anti-inflammatory medicine screening.
- Subjects :
- Adult
Aspirin pharmacology
Cell Line, Tumor
Cytokines metabolism
Female
Humans
Inflammation
Intramolecular Oxidoreductases metabolism
Male
Middle Aged
Nitrobenzenes pharmacology
Prostaglandin-E Synthases
RNA, Small Interfering metabolism
Sulfonamides pharmacology
Transfection
Influenza A virus metabolism
Interleukins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 3
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 18414668
- Full Text :
- https://doi.org/10.1371/journal.pone.0001985