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Polyamines and salt stress response and tolerance in Arabidopsis thaliana flowers.
- Source :
-
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2008 May-Jun; Vol. 46 (5-6), pp. 607-13. Date of Electronic Publication: 2008 Mar 04. - Publication Year :
- 2008
-
Abstract
- In the present study we analysed polyamine metabolism in Arabidopsis thaliana (ecotype Columbia) flowers and stalks collected from plants germinated and grown under increasing salt-stress conditions (0-75 mM NaCl). The expression level of the different isoforms of polyamine biosynthetic enzymes was analysed by reverse transcriptase-polymerase chain reaction (RT-PCR). Spermidine synthase enzyme activity determined both in supernatant and pellet fractions, together with RT-PCR results, led us to hypothesize a different intracellular compartmentation of the isoforms of these enzymes. Free and conjugated polyamines (perchloric acid-soluble and -insoluble) were measured. Free spermidine was the most abundant polyamine and its levels, such as those of free spermine, increased with salt concentration, supporting the hypothesis for a specific role of those polyamines in the response and tolerance to salt stress of Arabidopsis thaliana flowers.
- Subjects :
- Adaptation, Physiological drug effects
Arabidopsis drug effects
Arabidopsis genetics
Flowers drug effects
Flowers genetics
Gene Expression Regulation, Plant drug effects
Putrescine metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spermidine metabolism
Spermidine Synthase genetics
Spermidine Synthase metabolism
Spermine metabolism
Arabidopsis metabolism
Flowers metabolism
Polyamines metabolism
Sodium Chloride pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0981-9428
- Volume :
- 46
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Plant physiology and biochemistry : PPB
- Publication Type :
- Academic Journal
- Accession number :
- 18434176
- Full Text :
- https://doi.org/10.1016/j.plaphy.2008.02.005