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Vaccinia viruses with mutations in the E3L gene as potential replication-competent, attenuated vaccines: scarification vaccination.
- Source :
-
Vaccine [Vaccine] 2008 Jun 02; Vol. 26 (23), pp. 2860-72. Date of Electronic Publication: 2008 Apr 08. - Publication Year :
- 2008
-
Abstract
- In this study, we evaluated the efficacy of vaccinia virus (VACV) containing mutations in the E3L virulence gene to protect mice against a lethal poxvirus challenge after vaccination by scarification. VACV strains with mutations in the E3L gene had significantly decreased pathogenicity, even in immune deficient mice, yet retained the ability to produce a potent Th1-dominated immune response in mice after vaccination by scarification, while protecting against challenge with wild type, pathogenic VACV. Initial experiments were done using the mouse-adapted, neurovirulent Western Reserve (WR) strain of vaccinia virus. Testing of the full E3L deletion mutation in the Copenhagen and NYCBH strains of VACV, which are more appropriate for use in humans, produced similar results. These results suggest that highly attenuated strains of VACV containing mutations in E3L have the potential for use as scarification administered vaccines.
- Subjects :
- Administration, Intranasal
Animals
Antibodies, Viral analysis
Antibodies, Viral biosynthesis
Antiviral Agents pharmacology
Cells, Cultured
Comet Assay
Cricetinae
Cytokines biosynthesis
Drug Resistance, Viral genetics
Female
Genes, Viral genetics
Genes, Viral immunology
Immunity, Cellular immunology
Interferons pharmacology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, SCID
Mutation genetics
Mutation immunology
Neutralization Tests
Rabbits
Spleen cytology
Spleen immunology
Vaccines, Attenuated administration & dosage
Vaccines, Attenuated genetics
Vaccines, Attenuated immunology
Viral Vaccines administration & dosage
Viral Vaccines immunology
Virus Replication
Vaccinia immunology
Vaccinia prevention & control
Vaccinia virus genetics
Viral Vaccines genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 26
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 18455281
- Full Text :
- https://doi.org/10.1016/j.vaccine.2008.03.044