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Expression of hypoxia-inducible factor 1alpha gene affects the outcome in patients with ovarian cancer.
- Source :
-
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society [Int J Gynecol Cancer] 2008 May-Jun; Vol. 18 (3), pp. 499-505. - Publication Year :
- 2008
-
Abstract
- We conducted study to determine whether and how the expression of the hypoxia-inducible factor 1alpha (HIF-1alpha) gene relates to outcome in patients with epithelial ovarian cancer. A total of 66 patients with epithelial ovarian cancer, who underwent primary surgery followed by platinum-based chemotherapy, were entered into this study. We confirmed the expression of HIF-1alpha and the vascular endothelial growth factor (VEGF) by immunohistochemistry. To determine the quantity of HIF-1alpha and VEGF expression, messenger RNA of each gene was measured by real-time reverse transcription-polymerase chain reaction. The cutoff values were determined by the receiver-operating characteristic curve according to survival. The protein expressions of HIF-1alpha and VEGF were strongly observed in the cancer cells. The cutoff value of HIF-1alpha and VEGF gene expression was 6.0 and 3.0, respectively. The expression of HIF-1alpha did not relate to clinical stage, but tumor with low VEGF expression was observed more frequently in stage I patients. The response rate to chemotherapy did not differ between high and low expression of both genes. The overall survival for patients with high expression of HIF-1alpha was significantly lower, but disease-free survival did not differ between high and low expression of HIF-1alpha, whereas both overall and disease-free survival for patients with high expression of VEGF were significantly lower. Multivariate analysis revealed that FIGO stage and HIF-1alpha expression were independent prognostic factors but that VEGF was not. The present study suggested that the expression level of HIF-1alpha could be an independent prognostic factor in epithelial ovarian cancer.
- Subjects :
- Adult
Aged
Biomarkers, Tumor genetics
Chemotherapy, Adjuvant
Combined Modality Therapy
Confidence Intervals
Female
Humans
Hypoxia-Inducible Factor 1, alpha Subunit genetics
Immunohistochemistry
Kaplan-Meier Estimate
Middle Aged
Multivariate Analysis
Neoplasm Staging
Neoplasms, Glandular and Epithelial mortality
Neoplasms, Glandular and Epithelial pathology
Neoplasms, Glandular and Epithelial therapy
Ovarian Neoplasms mortality
Ovarian Neoplasms pathology
Ovarian Neoplasms therapy
Ovariectomy methods
Probability
Prognosis
Proportional Hazards Models
Prospective Studies
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Treatment Outcome
Vascular Endothelial Growth Factor A genetics
Gene Expression Regulation, Neoplastic
Hypoxia-Inducible Factor 1, alpha Subunit metabolism
Neoplasms, Glandular and Epithelial genetics
Ovarian Neoplasms genetics
Vascular Endothelial Growth Factor A metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1525-1438
- Volume :
- 18
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of gynecological cancer : official journal of the International Gynecological Cancer Society
- Publication Type :
- Academic Journal
- Accession number :
- 18476949
- Full Text :
- https://doi.org/10.1111/j.1525-1438.2007.01055.x