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Assessments of lung digestion methods for recovery of fibers.
- Source :
-
Environmental research [Environ Res] 1991 Apr; Vol. 54 (2), pp. 183-93. - Publication Year :
- 1991
-
Abstract
- Evaluation of the pulmonary hazards associated with exposure to fibrous materials tends to be more complicated than assessments required for particulate materials. Fibers are defined by aspect ratios and it is generally considered that physical dimensions play an important role in the pathogenesis of fiber-related lung diseases. Several digestion techniques have been used to recover fibers from exposed lung tissue for clearance studies. Because many of the digestion fluids are corrosive (e.g., bleach, KOH), it is conceivable that the dimensions of recovered fibers are modified during the tissue digestion methods to assess whether the physical dimensions of bulk samples of fibers were altered following simulated digestion processing. Aliquots of crocidolite and chrysotile asbestos, Kevlar aramid, wollastonite, polyacrylonitrile (pan)-based carbon, and glass fibers were incubated with either saline, bleach, or KOH and then filtered. Scanning electron microscopy techniques were utilized to measure the physical dimensions (i.e., lengths and diameters) of at least 160 fibers per treatment group of each fiber type. Our results showed that the lengths and diameters of glass fibers and wollastonite were altered after treatment with KOH. In addition, treatment with bleach produced a small reduction in both asbestos fiber-type diameters, and greater changes in Kevlar and wollastonite diameters and carbon fiber lengths (P less than 0.05). These results indicate that lung digestion methods should be carefully assessed for each fiber type before initiating fiber clearance studies.
- Subjects :
- Asbestos adverse effects
Carbon adverse effects
Carbon Fiber
Digestion
Humans
Hydroxides pharmacology
Hypochlorous Acid pharmacology
Lung drug effects
Lung ultrastructure
Lung Diseases chemically induced
Lung Diseases pathology
Microscopy, Electron, Scanning
Polymers adverse effects
Potassium pharmacology
Silicic Acid adverse effects
Sodium Chloride pharmacology
Asbestos analysis
Calcium Compounds
Carbon analysis
Glass analysis
Lung chemistry
Polymers analysis
Potassium Compounds
Silicates
Silicic Acid analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0013-9351
- Volume :
- 54
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Environmental research
- Publication Type :
- Academic Journal
- Accession number :
- 1851478
- Full Text :
- https://doi.org/10.1016/s0013-9351(05)80100-8