Atrial natriuretic peptide stability.

Authors :: Zolty R
Bauer C
Allen P
Garrity M
Vittorio TJ
Source :: Clinical biochemistry [Clin Biochem] 2008 Oct; Vol. 41 (14-15), pp. 1255-8. Date of Electronic Publication: 2008 May 27.
Publication Year :: 2008
Abstract: Objective: Atrial natriuretic peptide (ANP) is a key regulator in the homeostasis of water excretion and has emerged as an important prognostic marker for symptomatic chronic heart failure (CHF). The stability of ANP represents a crucial factor in assessing its use as a cardiac biomarker. Accordingly, we assessed the stability of ANP in blood samples collected from healthy controls and CHF subjects for a 12 month period.<br />Methods: Blood samples from 10 healthy controls and 12 symptomatic CHF subjects with left ventricular systolic dysfunction were drawn. Determination of plasma ANP was performed by a standardized radioimmunoassay protocol.<br />Results: The ANP levels of healthy subjects were 68.5+/-11.6 pg/mL at baseline and 69.9+/-17.2 pg/mL at 12 months (p=0.71). The ANP concentrations of CHF subjects were 199.25+/-44.8 pg/mL at baseline and 197.83+/-47.4 pg/mL at 12 months (p=0.70) respectively.<br />Conclusion: ANP is a stable molecule with no evidence of degradation when stored at -80 degrees C.