Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe(3)O(4)@SiO(2) nanoparticles: application to comprehensive proteomic profiling.

Authors :: Palani A
Lee JS
Huh J
Kim M
Lee YJ
Chang JH
Lee K
Lee SW
Source :: Journal of proteome research [J Proteome Res] 2008 Aug; Vol. 7 (8), pp. 3591-6. Date of Electronic Publication: 2008 Jun 19.
Publication Year :: 2008
Abstract: Superparamagnetic Fe(3)O(4)@SiO(2) core-shell nanoparticles (ca. 30 nm diameter), which are surface-modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.

Subjects :: Chromatography, Liquid
Indicators and Reagents
Peptides isolation & purification
Tandem Mass Spectrometry
Cysteine analysis
Ferrosoferric Oxide
Nanoparticles
Peptides analysis
Proteomics methods
Silicon Dioxide
Succinimides

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