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Early separation of B and T lymphocyte precursors in chick embryo.
- Source :
-
The Journal of experimental medicine [J Exp Med] 1991 Aug 01; Vol. 174 (2), pp. 397-406. - Publication Year :
- 1991
-
Abstract
- Embryonic chimeras were used to demonstrate an early separation of chicken T and B cell precursors. Genetically polymorphic cell surface antigens, Bu-1 and Ov, which are expressed on cells of the B and T lineage, respectively, are useful markers in adoptive cell transfer studies. Allelic products Bu-1a and Bu-1b can be detected with monoclonal antibodies (mAbs) L22 and 11G2, respectively, and the Ov antigen with mAb 11A9. Chimeric chickens were constructed by reconstituting irradiated 14-d Ov- H.B19 embryos with the sorted Bu-1+ or Bu-1- fractions of spleen cells from age-matched H.B19 Ov+ embryos. Chimeras were analyzed, 3-4 wk after hatching, for the presence of Ov+ cells in the bursa, thymus, spleen, and peripheral blood lymphocytes. T cell precursors giving rise to thymocytes and peripheral T cells were present only in the Bu-1-, but not in the Bu-1+, fraction. We previously demonstrated that, in contrast, all B cell precursors in spleen from 14-d embryos are exclusively present in the Bu-1+ fraction. We also analyzed the immunoglobulin light chain gene rearrangement in these populations by polymerase chain reaction. We show here that VJ recombination occurs in the Bu-1+, but not in the Bu-1-, fraction of spleen. These data demonstrate an early commitment to the B cell lineage, which occurs before the colonization of the bursa of Fabricius. Segregation of B cell precursors from the other hemopoietic precursors, and consequently separation of T and B cell precursors, occurs before the colonization of the primary lymphoid organs.
- Subjects :
- Alleles
Animals
Antibodies, Monoclonal immunology
Antigens, Surface immunology
B-Lymphocytes immunology
Base Sequence
Cell Differentiation
Chick Embryo
Chickens
Chimera
DNA analysis
Fluorescent Antibody Technique
Gene Rearrangement, B-Lymphocyte, Light Chain immunology
Immunoenzyme Techniques
Lymphoid Tissue cytology
Molecular Sequence Data
Oligonucleotide Probes
Polymerase Chain Reaction
Stem Cells cytology
T-Lymphocytes immunology
B-Lymphocytes cytology
T-Lymphocytes cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 174
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 1856628
- Full Text :
- https://doi.org/10.1084/jem.174.2.397