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Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery.

Authors :
Lee HJ
Kang MJ
Lee EY
Cho SY
Kim H
Paik YK
Source :
Proteomics [Proteomics] 2008 Aug; Vol. 8 (16), pp. 3371-81.
Publication Year :
2008

Abstract

A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.

Details

Language :
English
ISSN :
1615-9861
Volume :
8
Issue :
16
Database :
MEDLINE
Journal :
Proteomics
Publication Type :
Academic Journal
Accession number :
18651672
Full Text :
https://doi.org/10.1002/pmic.200800111