Back to Search
Start Over
Deficiency of ribosomal protein S19 during early embryogenesis leads to reduction of erythrocytes in a zebrafish model of Diamond-Blackfan anemia.
- Source :
-
Human molecular genetics [Hum Mol Genet] 2008 Oct 15; Vol. 17 (20), pp. 3204-11. Date of Electronic Publication: 2008 Jul 24. - Publication Year :
- 2008
-
Abstract
- Ribosomes are responsible for protein synthesis in all cells. Ribosomal protein S19 (RPS19) is one of the 79 ribosomal proteins (RPs) in vertebrates. Heterozygous mutations in RPS19 have been identified in 25% of patients with Diamond-Blackfan anemia (DBA), but the relationship between RPS19 mutations and the pure red-cell aplasia of DBA is unclear. In this study, we developed an RPS19-deficient zebrafish by knocking down rps19 using a Morpholino antisense oligo. The RPS19-deficient animals showed a dramatic decrease in blood cells as well as deformities in the head and tail regions at early developmental stages. These phenotypes were rescued by injection of zebrafish rps19 mRNA, but not by injection of rps19 mRNAs with mutations that have been identified in DBA patients. Our results indicate that rps19 is essential for hematopoietic differentiation during early embryogenesis. The effects were specific to rps19, but knocking down the genes for three other RPs, rpl35, rpl35a and rplp2, produced similar phenotypes, suggesting that these genes might have a common function in zebrafish erythropoiesis. The RPS19-deficient zebrafish will provide a valuable tool for investigating the molecular mechanisms of DBA development in humans.
- Subjects :
- Anemia, Diamond-Blackfan genetics
Animals
Base Sequence
DNA Primers genetics
Disease Models, Animal
Erythropoiesis genetics
Gene Targeting
Humans
Mutation
Phenotype
RNA, Antisense genetics
RNA, Messenger biosynthesis
RNA, Messenger genetics
Ribosomal Proteins blood
Ribosomal Proteins genetics
Zebrafish genetics
Zebrafish Proteins blood
Anemia, Diamond-Blackfan blood
Anemia, Diamond-Blackfan embryology
Ribosomal Proteins deficiency
Zebrafish blood
Zebrafish embryology
Zebrafish Proteins deficiency
Zebrafish Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2083
- Volume :
- 17
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Human molecular genetics
- Publication Type :
- Academic Journal
- Accession number :
- 18653748
- Full Text :
- https://doi.org/10.1093/hmg/ddn216