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Transposition of Mboumar-9: identification of a new naturally active mariner-family transposon.
- Source :
-
Journal of molecular biology [J Mol Biol] 2008 Oct 10; Vol. 382 (3), pp. 567-72. Date of Electronic Publication: 2008 Jul 23. - Publication Year :
- 2008
-
Abstract
- Although mariner transposons are widespread in animal genomes, the vast majority harbor multiple inactivating mutations and only two naturally occurring elements are known to be active. Previously, we discovered a mariner-family transposon, Mboumar, in the satellite DNA of the ant Messor bouvieri. Several copies of the transposon contain a full-length open reading frame, including Mboumar-9, which has 64% nucleotide identity to Mos1 of Drosophila mauritiana. To determine whether Mboumar is currently active, we expressed and purified the Mboumar-9 transposase and demonstrate that it is able to catalyze the movement of a transposon from one plasmid to another in a genetic in vitro hop assay. The efficiency is comparable to that of the well-characterized mariner transposon Mos1. Transposon insertions were precise and were flanked by TA duplications, a hallmark of mariner transposition. Mboumar has been proposed to have a role in the evolution and maintenance of satellite DNA in M. bouvieri and its activity provides a means to examine the involvement of the transposon in the genome dynamics of this organism.
- Subjects :
- Animals
Base Sequence
DNA, Satellite genetics
DNA-Binding Proteins genetics
Evolution, Molecular
Insect Proteins genetics
Molecular Sequence Data
Plasmids genetics
Plasmids metabolism
Sequence Alignment
Transposases genetics
Ants genetics
Ants metabolism
DNA Transposable Elements genetics
DNA-Binding Proteins metabolism
Genes, Insect
Insect Proteins metabolism
Transposases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8638
- Volume :
- 382
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 18675277
- Full Text :
- https://doi.org/10.1016/j.jmb.2008.07.044