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Gastric cancer cell lines induced by trichostatin A.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2008 Aug 14; Vol. 14 (30), pp. 4810-5. - Publication Year :
- 2008
-
Abstract
- Aim: To explore the effect of trichostatin A (TSA) on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901.<br />Methods: The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot.<br />Results: TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups (37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line) and control group (0.85+/-0.14 vs 1.14+/-0.07, P=0.02; 0.94+/-0.07 vs 1.15+/-0.06, P=0.02). Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of G1-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group.<br />Conclusion: TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.
- Subjects :
- Acetylation
Cell Line, Tumor
Cell Proliferation drug effects
Dose-Response Relationship, Drug
Histone Deacetylases metabolism
Humans
Stomach Neoplasms enzymology
Time Factors
Antineoplastic Agents pharmacology
Apoptosis drug effects
Enzyme Inhibitors pharmacology
Histone Deacetylase Inhibitors
Histones metabolism
Hydroxamic Acids pharmacology
Stomach Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 14
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 18720545
- Full Text :
- https://doi.org/10.3748/wjg.14.4810