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Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths.
- Source :
-
The ISME journal [ISME J] 2009 Jan; Vol. 3 (1), pp. 47-64. Date of Electronic Publication: 2008 Sep 04. - Publication Year :
- 2009
-
Abstract
- Bacterial community succession was investigated in a field-scale subsurface reactor formed by a series of wells that received weekly ethanol additions to re-circulating groundwater. Ethanol additions stimulated denitrification, metal reduction, sulfate reduction and U(VI) reduction to sparingly soluble U(IV). Clone libraries of SSU rRNA gene sequences from groundwater samples enabled tracking of spatial and temporal changes over a 1.5-year period. Analyses showed that the communities changed in a manner consistent with geochemical variations that occurred along temporal and spatial scales. Canonical correspondence analysis revealed that the levels of nitrate, uranium, sulfide, sulfate and ethanol were strongly correlated with particular bacterial populations. As sulfate and U(VI) levels declined, sequences representative of sulfate reducers and metal reducers were detected at high levels. Ultimately, sequences associated with sulfate-reducing populations predominated, and sulfate levels declined as U(VI) remained at low levels. When engineering controls were compared with the population variation through canonical ordination, changes could be related to dissolved oxygen control and ethanol addition. The data also indicated that the indigenous populations responded differently to stimulation for bioreduction; however, the two biostimulated communities became more similar after different transitions in an idiosyncratic manner. The strong associations between particular environmental variables and certain populations provide insight into the establishment of practical and successful remediation strategies in radionuclide-contaminated environments with respect to engineering controls and microbial ecology.
- Subjects :
- Bacteria metabolism
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Ethanol metabolism
Genes, rRNA
Metals metabolism
Oxidation-Reduction
Phylogeny
RNA, Bacterial genetics
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sulfates metabolism
Bacteria classification
Bacteria isolation & purification
Biodegradation, Environmental
Biodiversity
Environmental Microbiology
Uranium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1751-7370
- Volume :
- 3
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The ISME journal
- Publication Type :
- Academic Journal
- Accession number :
- 18769457
- Full Text :
- https://doi.org/10.1038/ismej.2008.77