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Rat mesenchymal stem cells increase tyrosine hydroxylase expression and dopamine content in ventral mesencephalic cells in vitro.
- Source :
-
Cell biology international [Cell Biol Int] 2008 Nov; Vol. 32 (11), pp. 1433-8. Date of Electronic Publication: 2008 Aug 20. - Publication Year :
- 2008
-
Abstract
- Mesenchymal stem cells (MSCs) are pluripotent adult stem cells. It has been shown that MSCs secrete neurotrophic factors involving nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). Also, these neurotrophic factors can upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells and neural stem cells. Here, we investigated the effect of co-culturing rat E13.5 ventral mesencephalic cells (VMCs) with MSCs from rat bone marrow on TH expression and dopamine (DA) content. The study consisted of 3 groups: MSC, VMC and a combined MSC+VMC group. All groups were cultured in serum-free neuro-basal medium for 3 days. Thereafter, each group was analyzed by RT-PCR, western blotting, and HPLC. The co-culture group showed a higher expression at TH and DA than the VMC group. However, TH and DA were not present in the MSC group. These observations suggest that MSCs could be an alternative source for treating neurodegenerative diseases such as Parkinson's disease (PD).
- Subjects :
- Animals
Cells, Cultured
Coculture Techniques
Male
Mesencephalon cytology
Mesenchymal Stem Cell Transplantation methods
Nerve Growth Factors metabolism
Neurons cytology
Parkinson Disease surgery
Rats
Rats, Sprague-Dawley
Substantia Nigra cytology
Substantia Nigra metabolism
Up-Regulation physiology
Ventral Tegmental Area cytology
Ventral Tegmental Area metabolism
Cell Communication physiology
Dopamine metabolism
Mesencephalon metabolism
Mesenchymal Stem Cells metabolism
Neurons metabolism
Tyrosine 3-Monooxygenase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8355
- Volume :
- 32
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Cell biology international
- Publication Type :
- Academic Journal
- Accession number :
- 18778785
- Full Text :
- https://doi.org/10.1016/j.cellbi.2008.08.014